SERUM TYROSYL KINASES IN HUMAN NEOPLASIA

酪氨酸特异性蛋白激酶是一种与 几种癌基因蛋白和生长因子受体。与此相关的蛋白质 罕见的活动已被证明会在动物身上引起肿瘤，并且 在一些人类肿瘤中被放大。我们的初步研究表明 血清是一种容易获得的人体标本，可用于研究 酪氨酸激酶在正常和肿瘤细胞中的表达 有机体。我们发现血清酪氨酸激酶活性是 在人类的发育中表达，并且活性在 某些肿瘤患者的血清。 我们的目标是：(1)鉴定血清酪氨酸激酶可能与 与人类肿瘤疾病有关；(2)鉴定血清酪氨酸激酶 可用于癌症诊断试验；以及(3)用于识别酪氨酸激酶 这与人类发展的不同阶段有关。要实现 我们将对这些目标进行细分，并对不同的酪氨酸进行描述 人血清中的激酶种类。血清酶与糖尿病的关系 生长因子受体和癌基因蛋白将通过以下方式建立 生化比较和对特定免疫学的反应 试剂。那些与之前无关的血清酪氨酸激酶 识别的酶将被自动磷酸化以识别活性 亚基分子量，糖基化测试，用于制备 抗体。将对特定的酪氨酸激酶和 他们在不同年龄段的血清中的活动水平来定义 在发育过程中表达的物种和患者的血清中 不同的肿瘤疾病来定义相关的物种 有肿瘤。肿瘤分期与酶水平的相关性研究 这一疾病将表明酪氨酸激酶检测在癌症中的价值 诊断。(1)

Tyrosine-specific protein kinase is an enzyme activity associated with several oncogene proteins and growth factor receptors. Proteins with this rare activity have been shown to cause tumors in animals and to be amplified in some human tumors. Our preliminary studies indicate that serum is an easily obtained human specimen which can be used to investigate tyrosyl kinases expressed in normal and neoplastic cells in the intact organism. We have found that serum tyrosyl kinase activity is developmentally expressed in humans and that the activity is elevated in sera from some patients with neoplastic disorders. Our aims are: (1)\to identify serum tyrosyl kinases that may be associated with human neoplastic disorders; (2)\to identify serum tyrosyl kinases that may be used in cancer diagnostic tests; and (3)\to identify tyrosyl kinases that are associated and different stages of human development. To achieve these aims we will fractionate and characterize the different tyrosyl kinase species in human serum. The relationship of serum enzymes with growth factor receptors and oncogene proteins will be established by biochemical comparisons and by reactivity to specific immunological reagents. Those serum tyrosyl kinases that are unrelated to previously identified enzymes will be autophosphorylated to identify the active subunit molecular weights, tested for glycosylation, and used to prepare antibodies. Comparisons will be made of the specific tyrosyl kinases and their levels of activity in sera from different age groups to define the species that are developmentally expressed and in sera from patients with different neoplastic disorders to define the species that are associated with neoplasia. Correlations of the levels of the enzyme with the stage of the disease will indicate the value of the tyrosyl kinase assay in cancer diagnosis. (1)