METABOLITES FROM TERTIARY AMINES--PHENCYCLIDINE

建议进行调查，以评估生物医学意义的 通过细胞色素P-450介导的代谢形成的亚胺中间体 叔胺 苯环利定将被用作模型化合物，以检查 这些亲电离子对生物大分子的反应性。 代谢依赖性抑制的生化机制 将研究苯环利定对细胞色素P-450的酶活性 使用体外肝微粒体制备物， 细胞色素P-450系统。 生物物种将被合成，它们的 将研究对模型化合物的反应性，并研究 形成的加合物将通过质谱方法表征。 程度 亚胺中间体的形成对组织是特异性的， 物种将通过使用14 CN捕获方法来确定。 亚胺中间体的形成及其反应的文献 与生物大分子的结合， 与叔胺的毒性作用有关，其中许多是 有用的治疗剂。

Investigations are proposed to assess the biomedical significance of iminium intermediates formed via cytochrome P-450 mediated metabolism of tertiary amines. Phencyclidine will be used as a model compound to examine the reactivity of these electrophilic ions towards biomacromolecules. Biochemical mechanisms underlying metabolism-dependent inhibition of cytochrome P-450 enzymic activities by phencyclidine will be investigated using hepatic microsomal preparations in vitro and reconstituted, purified cytochrome P-450 systems. Iminium species will be synthesized, their reactivity towards model compounds will be studied, and the structure of adducts formed will be characterized by mass spectral methods. The extent to which the formation of iminium intermediates is specific to tissues and species will be determined by use of 14CN- trappping methods. Documentation of the formation of iminium intermediates and their reactions with biomacromolecules should provide insight into the molecular mechanisms associated with the toxic actions of tertiary amines, many of which are useful therapeutic agents.