EARLY EVENTS--CHEMICAL CARCINOGEN INDUCED MAMMARY TUMOR

这项拨款申请的长期目标是了解 乳腺癌前病变的细胞和分子基础 转型。白纹伊蚊的基本生物学特性 然而，对乳腺增生症的描述已经很充分。 这些特性的细胞和分子相关性尚不清楚。 最近对生长和生长的适当方法的描述 体外化学转化乳腺上皮细胞的实验研究 对原生物质的存在和性质的爆炸性记录- 癌基因为理解长期追寻的人提供了新的途径 目标。在此应用程序中，目标是检查特定的 细胞和分子特性，这可能是 乳腺增生症的观察特征。具体来说， 乳腺上皮细胞将在体外转化为 胶原蛋白-细胞外基质凝胶和细胞检测 体内转化特性。经过处理的细胞将被 检查生长因子依赖的变化，原- 癌基因表达(那些同源的原癌基因 激素受体)，角蛋白表达的改变以及 创业型的形态类型。

The long-term objective of this grant application is to understand the cellular and molecular basis for mammary gland preneoplastic transformation. The essential biological characteristics of mammary preneoplasias have been adequately described, however the cell and molecular correlates of these properties are unknown. The recent description of appropriate methods to grow and chemically transform mammary epithelial cells in vitro and the explosive documentation of the existence and properties of proto- oncogenes provide new avenues to understand long sought after goals. In this application, the aims are to examine specific cellular and molecular properties which may be the basis for the observed properties of mammary preneoplasias. Specifically, mammary epithelial cells will be transformed in vitro in a collagen-extracellular matrix gel and the cells assayed for transformation properties in vivo. The treated cells will be examined for alterations in growth factor dependence, proto- oncogene expression (those proto-oncogenes that are homologues of hormone receptors), altered keratin expression as well as morphological types of preneoplasias.