CELLULAR GENETIC ASPECTS OF OPIATE USE

作为评估阿片剂滥用的长期遗传影响的计划的一部分， 研究替代药物或拮抗剂的改善，一系列压力 已经开发了系统来测试电池处理电势的能力 诱变危机。海洛因成瘾者染色体频率增加 非应激白细胞培养和应激系统中的损伤 表明它们修复远紫外线损伤的能力也存在缺陷，因为 通过程序外DNA合成和姐妹染色单体的改变来测量 紫外线和丝裂霉素C应激时的交换反应 诱导的DNA修复和染色体畸变在长期后得到纠正 美沙酮维持。为了进一步理解这些发现的含义 研究计划用第二个测试系统来评估紫外线损伤的修复 也可以使用化学应激剂来代替紫外线。它的速度是 美沙酮逆转生化和细胞遗传学效应 将测定化学和紫外线压力，并与发现的比率进行比较 用于LAAM和纳曲酮。这些研究应该有助于确定 这些治疗方式在遗传水平上的相对有效性。在……里面 结合体内实验研究，还将启动 尝试用成纤维细胞培养在体外复制这些发现 研究产生这些效应的机制。

As part of a program to evaluate long term genetic effects of opiate abuse and investigate amelioration with replacements or antagonists, a series of stress systems has been developed to test the capacity of the cell to handle potential mutagenic crises. Heroin addicts have an increased frequency of chromosome damage in unstressed leukocyte cultures and with the stress systems it can be shown that there is also a defect in their ability to repair far UV damage as measured by unscheduled DNA synthesis and an alteration in sister chromatid exchange response when stressed by UV and mitomycin C. The changes in UV induced DNA repair and chromosome aberrations were corrected after long term methadone maintenance. To further understand the implication of these findings studies are planned to evaluate repair of UV damage with a second assay system and also to use a chemical stress agent in place of UV. The rate at which methadone reverses both the biochemical and cytogenetic effects as measured with chemical and UV stresses will be determined and compared with the rates found for LAAM and naltrexone. These studies should be useful in determining the relative effectiveness of these treatment modalities at the genetic level. In conjunction with the in vivo experiments studies will also be initiated to attempt to reproduce these findings in vitro with fibroblast cultures to investigate the mechanism which produce these effects.