13TSB_N4L2: CELLDEX - Development of a low calorie bulk sugar replacer

RINH的微生物学小组（U。Aberdeen）将执行TSB项目CELLDEX内工作包3的关键要素（WP3.1-“体外人类结肠细菌对纤维糊精可降解性的评估”）。他们将使用体外方法来评估人体肠道微生物对纤维糊精的发酵能力，并预测纤维糊精对微生物群落组成和代谢产物的可能影响。在厌氧条件下，使用分批培养和pH控制的连续流动发酵罐模型，将纤维糊精与混合的人类粪便微生物群一起孵育。分子方法将用于监测微生物群落组成的变化。发酵的主要产物短链脂肪酸和气体将通过气相色谱法进行监测，纤维糊精的利用将通过总糖分析进行监测。我们还将评估我们实验室从人结肠中分离出的一系列主要利用多糖的厌氧菌以纤维糊精作为唯一能源生长的能力。与分子群落分析结果一起，这应该提供可能负责纤维糊精利用的物种的信息。这项工作将先于诺丁汉大学计划进行的人类饮食干预研究，并为之提供信息。

The Microbiology Group at RINH (U. Aberdeen) will perform a key element (WP3.1- "Assessment of cellodextrinfermentability by human colonic bacteria in vitro") of Work package 3 within the TSB project CELLDEX. They will use invitro approaches to assess the fermentability of cellodextrins by human intestinal microorganisms and to predict the likelyimpact of cellodextrins upon microbial community composition and metabolic outputs. Cellodextrins will be incubated withmixed human faecal microbiota under anaerobic conditions using batch cultures and pH-controlled continuous flowfermentor models. Molecular approaches will be used to monitor changes in microbial community composition. The majorproducts of fermentation, short chain fatty acids and gases, will be monitored by gas chromatography and cellodextrinutilization will be monitored by analysis of total sugars. The ability of a range of dominant polysaccharide-utilizinganaerobes that have been isolated in our laboratory from from the human colon to grow with cellodextrins as a sole energysource will also be assessed here. Together with the results from molecular community analysis, this should provideinformation on species likely to be responsible for cellodextrin utilization. This work will precede and inform the plannedhuman dietary intervention studies to be performed by University of Nottingham.