ULTRASTRUCTURE OF EXPERIMENTAL PERIODONTITIS

我们计划进行免疫细胞化学研究，定位纤维连接蛋白（FN） 正常比格犬牙周组织中Ⅰ型和Ⅲ型胶原（Col Ⅰ，Col Ⅲ） 并确定在结扎诱导和 自然发生的牙周炎 我们将测量FN、Col I和Col III 并将这些水平与炎症相关联， 通过光镜、电镜和免疫细胞化学测定。 使用 同样的方法，我们将研究抑制胶原酶的效果， 米诺环素对龈沟液FN、Col I、Col III水平的影响 液 我们希望表明，监测FN，Col I和Col III， 龈沟液可以是一种准确的方法来衡量正在进行的组织 杀伤性 如果小猎犬的研究结果令人鼓舞，我们将开始 在人类中进行类似的研究。 在相关项目中，我们将使用 免疫细胞化学方法研究牙龈FN的存在 牙菌斑和牙结石。 我们将继续放射自显影研究的渗透和 应用的小代谢物掺入牙龈上皮细胞 牙龈沟区域。 我们希望确定细胞间是否 通讯和胞质内转运是一个重要的途径， 牙龈上皮 初步研究结果表明， 似乎值得。 在此期间，我们进行了 胶原蛋白分泌和成纤维细胞结构的大量基础研究。 我们建议通过定位成纤维细胞来继续这些重要的研究 通过荧光显微镜和抗肌动蛋白， 抗波形蛋白抗体的中间丝。 我们亦会研究 FN的分布与纤维粘连形成的关系 电子显微镜免疫细胞化学在以前的领域接触 通过光学显微镜鉴定为应力纤维丰富的部位 发展 我们希望将它们的存在与抗压力联系起来 在牙周结缔组织中。 还计划进行体外研究 进一步评估牙周膜的极性和运动 成纤维细胞与应力纤维的存在和分布 和纤连蛋白。

We plan to conduct immunocytochemical studies to localize fibronectin (FN) and collagen types I and III (Col I, Col III) in normal beagle periodontium and to determine changes in their distribution during ligature-induced and naturally-occurring periodontitis. We will measure FN, Col I, and Col III in gingival sulcular fluid and correlate these levels with inflammation as determined by light and electron microscopy and immunocytochemistry. Using the same methods, we will study the effect of inhibiting collagenase with minocycline on the level of FN, Col I, and Col III in gingival sulcular fluid. We expect to show that monitoring FN, Col I, and Col III in gingival crevicular fluid can be an accurate way to measure ongoing tissue destruction. If the beagle studies prove encouraging, we will begin similar investigations in humans. In a related project, we will use immunocytochemical methods to investigate the presence of FN in gingival plaque and calculus. We will continue autoradiographic studies of the penetration and incorporation into gingival epithelial cells of small metabolites applied to the gingival sulcular area. We hope to determine whether cell-to-cell communication and intracytoplasmic transport is a significant pathway in gingival epithelium. Preliminary findings suggest that further studies appear worthwhile. During the tenure of this grant, we have conducted numerous and basic studies of collagen secretion and fibroblast structure. We propose to continue these important studies by localizing fibroblasts rich in stress fibers by fluorescent microscopy with anti-actin and intermediate filaments with anti-vimentin antibodies. We will also examine the distribution of FN in relation to the development of fibronexus contacts by electron microscopy immunocytochemistry in areas previously identified by light microscopy to be sites rich in stress fiber development. We hope to correlate their presence with resistance to stress in the periodontal connective tissues. In vitro studies are also planned to further evaluate the polarity and locomotion of periodontal ligament fibroblasts in relation to the presence and distribution of stress fibers and fibronectin.