PHYSIOLOGY OF NEUROPHYSIN SECRETION

描述（改编自申请人的摘要）：在以前的 在研究中，申请人已经证明，精氨酸的基础水平 加压素（AVP）mRNA在成人中是稳定的， 与合成紧密结合。相反，他发现在青春期前， 大鼠（断奶和性成熟之间）有一个渐进的 下丘脑AVP mRNA和垂体AVP均增加。 因此，委员会认为， 他假设内分泌平衡发生了两个显著的变化， 以确定成人AVP信息和垂体 内容：与断奶相关的钠和液体摄入量变化， 开始分泌性腺类固醇。该提案有5项具体内容 目的： 1.定量下丘脑AVP mRNA的动态变化， 垂体加压素含量并确定细胞机制 调节成熟大鼠AVP的合成。 2.在幼鼠体内， 确定渗透操作对加压素合成的影响， 垂体AVP的积累和确定的网站的调节 这些影响。 3.确定性腺类固醇对 垂体AVP合成和神经垂体AVP库的发育 未成熟大鼠4. 研究渗透压和类固醇输入的相互作用 加压素合成的调节，并确定它们是否 是独立的和附加的，允许的，或协同的。 5. 确定 长期影响的渗透和类固醇的操作过程中， 成熟期对加压素合成和神经叶AVP的影响 成年大鼠的内容。

DESCRIPTION (adapted from the applicant's abstract): In previous studies, the applicant has demonstrated that basal levels of arginine vasopressin (AVP) mRNA are stable in adults and that secretion is tightly coupled to synthesis. In contrast, he found that in prepubertal rats (between weaning and sexual maturity) there is a progressive increase in both hypothalamic AVP mRNA and pituitary AVP. Accordingly, he hypothesizes that two dramatic changes in endocrine balance occurs in development to determine adult levels of AVP message and pituitary content: changes in sodium and fluid intake associated with weaning and onset of secretion of gonadal steroids. This proposal has 5 Specific Aims: 1. Quantify the dynamics of hypothalamic AVP mRNA and pituitary vasopressin content and identify the cellular mechanism(s) regulating AVP synthesis in the maturing rat. 2. In the young rat, determine the impact of osmotic manipulation on vasopressin synthesis and pituitary AVP accumulation and determine the site of regulation of these effects. 3. Determine the influence of gonadal steroids on AVP synthesis and the development of neurohypophyseal AVP stores in the immature rat. 4. Study the interaction of osmotic and steroidal inputs to the regulation of vasopressin synthesis and determine whether they are independent and additive, permissive, or synergistic. 5. Determine the long-term impact of osmotic and steroidal manipulations during the maturational period on vasopressin synthesis and neural lobe AVP content in the adult rat.