ACTION OF HORMONES ON PROTEIN AND RNA METABOLISM

提供对雄激素正常作用途径的洞察 胚胎和胚胎后生命的各种方式和损害 关于雄激素作用会导致疾病状态的各种实验 都是有计划的。雄激素受体的抗独特型抗体将是 是为了更准确地描述损害人类健康的突变 雄激素受体及雄激素个体步骤的研究 将在正常和突变的成纤维细胞株中采取行动，包括 双氢睾酮-雄激素转化的研究 受体复合体(DHR-R)对DNA结合状态、DHT-R的相互作用 与核基质、受体后事件中雄激素的作用有关。 将研究男性泌尿生殖道的表型分化。 其具体目标是确定沃尔夫管是否男性化 直接由于雄激素的作用或间接由于某些 激素对睾丸本身的影响。细菌的分子生物学研究进展 5α-还原酶缺乏症将通过尝试开发一种 5α-还原酶的有效抗体，通过研究其作用 5α-还原酶抑制对男性泌尿生殖系统发育的影响 通过研究雄激素是如何促进精子发生的。 将研究作为内分泌器官的性腺的胚胎分化。 在大鼠和负鼠性腺的器官培养制剂中以及在小鼠中 携带一条X染色体或性反转突变。这个 外周组织中芳香酶反应的遗传控制 以鸡的母鸡羽化突变为模型的研究 卵巢正常发育和腺外检查系统 雌激素的形成。最后，我们将在 受体后水平，研究甲状腺激素和DHT相互作用的性质 在小鼠颌下腺中，对雄激素的独特抵抗作用 在某些野生来源的小鼠的肾脏中，以及对 小鼠L细胞中雄激素依赖的基因。

To provide insight into the normal pathway of androgen action during embryonic and postembryonic life and into the various ways that impairment of androgen action can result in disease states, a variety of experiments are planned. Anti-idiotypic antibodies to the androgen receptor will be made for the purpose of characterizing more precisely mutations that impair the androgen receptor, and studies of the individual steps in androgen action will be made in normal and mutant fibroblast strains, including investigation of the transformation of the dihydrotestosterone-androgen receptor complex (DHR-R) to the DNA-binding state, the interaction of DHT-R with the nuclear matrix, and the post receptor events in androgen action. Phenotypic differentiation of the male urogenital tract will be studied with the specific goal of determining whether the wolffian ducts virilize directly as a result of androgen action or indirectly as the result of some effect of the hormone on the testis itself. The molecular biology of 5Alpha-reductase deficiency will be investigated by attempting to develop a potent antibody to the 5Alpha-reductase enzyme, by investigating the effect of 5Alpha-reductase inhibition on the development of the male urogenital tract, and by studying how androgens act to promote spermatogenesis. Embryonic differentiation of the gonads as endocrine organs will be studied in organ culture preparations of rat and opossum gonads and in mice carrying either a single X chromosome or the sex reversal mutation. The genetic control of the aromatase reaction in peripheral tissue will be studied utilizing the henny feathering mutation in chickens as a model system for investigating normal ovarian development and extraglandular estrogen formation. Finally, we will explore androgen action at the post-receptor level, studying the nature of thyroxine and DHT interaction in the mouse submandibular gland, the unique resistance to androgen action in kidneys of certain wild-derived mouse strains, and the regulation of androgen-dependent genes in mouse L cells.