STUDIES ON THE SPHINCTER OF ODDI AND GALLBLADDER

这项建议的主要目标是研究神经激素 参与Oddi括约肌（SO）调节的机制， 胆囊（GB）运动功能和生理相互作用 在十二指肠和胆道之间 这些研究报告将 在体外肌肉浴中和在体内分离的 猫的尿道-胆道准备。 SO、GB和十二指肠运动 功能将通过测量机械和肌电 活动 我们的主要兴趣领域是：1）分析 在基础和刺激状态期间的肌电模式，以确定 机电耦合和解离的程度及其对 SO流速; 2）十二指肠受体的药理学特征 激素和神经递质，可能参与生理 控制基础或刺激的胆道运动功能; 3）我们将 扩展我们对内在神经通路性质的研究， 显示胆道的外部神经支配，以使 研究十二指肠和小肠之间的反射机制是可能的。 胆道; 4）我们将研究神经激素的关系，以确定 十二指肠激素（CCK，胃动素）和 迷走神经和交感神经。 这些研究将使我们能够检查 可能的相互作用类型，即增强或拮抗作用， 将增加我们对什么是生理刺激的理解， 开发胆道功能障碍的模型; 5）我们将继续这些研究 通过进一步检查这些神经激素的关系， 通过刺激内源性十二指肠激素的释放 和/或反射的激活。

The broad objectives of this proposal are to investigate the neurohormonal mechanisms involved in the regulation of the sphincter of Oddi (SO) and gallbladder (GB) motor function and in the physiological interaction between the duodenum and the biliary tract. These studies will be performed in vitro in the muscle bath and in vivo in an isolated duodenal-biliary tract preparation in cats. SO, GB, and duodenal motor function will be determined by measuring mechanical and myoelectrical activity. Our major areas of interest are: 1) analysis of the myoelectrical patterns during basal and stimulated states to determine the extent of electro-mechanical coupling and dissociation and their effects on SO flow rates; 2) pharmacological characterization of receptors of duodenal hormones and neurotransmitters which may participate in the physiological control of the basal or stimulated biliary tract motor function; 3) we will extend our studies of the nature of the intrinsic neural pathways and demonstrate the extrinsic innervation of the biliary tract in order to make possible the study of reflex mechanisms between the duodenum and the biliary tract; 4) we will investigate neurohormonal relationships to define the nature of the interaction between duodenal hormones (CCK, motilin) and the vagus and sympathetic nerves. These studies will permit us to examine possible types of interaction, that is, of potentiation or antagonism which will increase our understanding of what is a physiological stimulus and to develop models of biliary dysfunction; and 5) we will pursue these studies further by examining these neurohormonal relationships under physiological conditions by stimulating the release of endogenous duodenal hormones and/or the activation of reflexes.