课题基金 / 基金详情

STRUCTURE OF BRANCHED CHAIN KETO ACID DEHYDROGENASE

STRUCTURE OF BRANCHED CHAIN KETO ACID DEHYDROGENASE
支链酮酸脱氢酶的结构
批准号：
3227113
负责人：
JOHN R SOKATCH
金额：
$9.48万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
项目状态：
已结题
起止时间：
1979-07-01 至 1987-08-31
来源：
https://reporter.nih.gov/project-details/3227113
关键词：
Pseudomonas aminoacid metabolism bacterial genetics biological models branched chain aminoacid cofactor electron microscopy enzyme complex enzyme induction /repression enzyme structure enzyme substrate complex gel electrophoresis gene expression genetic mapping maple syrup urine disease microorganism metabolism molecular weight mutant orphan disease /drug radiotracer stoichiometry

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中文摘要
翻译
恶臭假单胞菌的支链酮脱氢酶是一种不常见的酶 其活性受到L-缬氨酸的刺激。 该酶已被纯化为 比活性为10单位/mg蛋白质。 纯化的酶含有4 分子量为49 k、46 k、39 k和37 k的亚单位。 49 k蛋白质 分离并鉴定为硫辛酰胺脱氢酶。 的目的 本年度的任务是分离和鉴定这些亚单位。 的 转酰酶亚基将通过其特异性标记来鉴定， N-乙基（2，3 - 14 C）马来酰亚胺在2-酮基异戊酸存在下。 的 脱氢酶（E1）亚基将通过其与铁氰化物的反应来鉴定 以及从2-酮（1- 14 C）异戊酸释放放射性二氧化碳。
英文摘要
Branched chain keto dehydrogenase of Pseudomonas putida is an unusual enzyme whose activity is stimulated by L-valine. The enzyme has been purified to a specific activity of 10 units/mg protein. The purified enzyme contains 4 subunis with molecular weights of 49k, 46k, 39k, and 37k. The 49k protein has been isolated and identified as lipoamide dehydrogenase. The objective of the current year will be to isolate and identify the remainding subunits. The transacylase subunit will be identified by its specific labeling with N-ethyl(2,3-14C) maleimide in the presence of 2-ketoisovalerate. The dehydrogenase (E1) subunit will be identified by its reaction with ferricyanide and the release of radioactive carbon dioxide from 2-keto(1-14C) isovalerate.
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