IMMUNE GLOMERULONEPHRITIS?

这些研究涉及内在的细胞之间的相互作用 免疫介导的肾小球细胞和浸润性免疫细胞 肾小球损伤。需要检查的肾小球疾病模型包括 抗肾小球基底膜和抗血清介导的肾小球肾炎 通过沉积免疫复合体。基本的实验方法 由酶解离受损的肾小球形成悬浮液 组成细胞(包括内源性和外源性)，分离 特定的细胞类型，以及它们的细胞活性在 短期组织培养和长期组织培养。特别强调的是 滞留和浸润性单核巨噬细胞的贡献。 将对肾小球内巨噬细胞进行特异性免疫检测 功能，如抗原提呈、分泌或展示 白细胞介素1与淋巴细胞趋化因子和花生四烯酸的分泌 酸性代谢物。内源性肾小球细胞的培养 检测其调节单核细胞向细胞募集的能力 肾小球。肾小球系膜细胞和上皮细胞将进一步评估 因为它们有能力调节巨噬细胞的免疫反应。这个 要检查的第一个参数将是Ia抗原的表达 因子作用下的肾小球内和腹膜巨噬细胞 由固有的肾小球细胞分泌。在平行研究中， 肾小球内和间质淋巴细胞将从免疫中获得 大鼠对特定抗原预致敏的肾脏损害 以及随后将该抗原放置在肾小球中。这个 淋巴细胞将通过单核抗体进行表型分类。 将对它们的激活状态进行评估，由 抗原驱动的增殖及其对免疫调节的释放 淋巴因子：白细胞介素2、干扰素和单核细胞趋化 各种因素。最后，体液和细胞免疫刺激对 肾小球固有细胞功能将通过肾小球系膜进行检查 血管紧张素II受体作为模型系统的细胞表达 化验过了。

The investigations concern the cellular interactions between intrinsic glomerular cells and infiltrating immune cells in immunologically mediated glomerular injury. Glomerular disease models to be examined include glomerulonephritis mediated by antisera to glomerular basement membrane and by deposition of immune complexes. The basic experimental approach consists of enzymatic disassociation of injured glomeruli into a suspension of constituent cells (both endogenous and exogenously derived), isolation of particular cell types, and the assay of their cellular activities in short-term and long-term tissue culture. Particular emphasis will be on the contributions of the resident ad infiltrating mononuclear phagocytes. Intraglomerular macrophages will be assayed for specifically immune functions such as antigen presentation, secretion or display of Interleukin-1, and secretion of lymphocyte chemoattractants and arachidonic acid metabolites. Cultures of endogenous glomerular cells are to be assayed for their capacity to regulate monocyte recruitment to the glomerulus. Mesangial cells and epithelial cells will be further evaluated for their ability to modulate the immunoreactivity of macrophages. The first parameter to be examined will be the expression of Ia antigens by intraglomerular and peritoneal macrophages in the presence of factors secreted by intrinsic glomerular cells. In parallel studies, intraglomerular and interstitial lymphocytes will be obtained from immune renal lesions involving pre-sensitization of rats to particular antigens and the subsequent placing of that antigen in the glomerulus. The lymphocytes will be phenotypically classified by mononuclear antibodies. They will be evaluated for their state of activation, as determined by antigen-driven proliferation and their release of the immunoregulatory lymphokines: Interleukin-2, gamma interferon, and monocyte chemotaxis factors. Finally, the effect of humoral and cellular immune stimuli on intrinsic glomerular cell function will be examined, with the mesangial cell expression of angiotensin II receptors as the model system to be assayed.