BENIGN PROSTATIC HYPERPLASIA AND PROSTATITIS

摘要良性前列腺增生症(BPH)是老年人的常见病。 男性。良性前列腺增生症和慢性前列腺炎在病理上都不准确。 已定义。前列腺炎，如本提案正文所述，一直是 被一位著名的泌尿科医生描述为临床的废纸篓 “无知。”这两种疾病状态(A)在 糖尿病患者和(B)涉及前列腺的变化，在水平上 组织的一部分，既有多样性也有多样性。试图理解 发病机制、生物化学、分子生物学、内分泌学和 这些损伤的免疫学将非常令人沮丧，直到准确 废纸篓中的许多不同实体的定义可以是 已定义。这项任务将通过形态测量来实现 对符合以下条件的解剖和病理结构进行量化 从20岁出头的男性开始观察。交替 每隔0.5厘米对整个前列腺进行连续切片 在石蜡和甲基丙烯酸酯中加工。对差异进行了修正 在组织收缩方面用这两种方法，计算机辅助的 将构建每个前列腺的三维图像。对.的使用 甲基丙烯酸酯允许卓越的原位杂交和演示 大多数酶和抗原。使用石蜡可以回收 适合用于聚合酶链式反应的核 以及用于流式细胞术。每个可识别的病变的频率将 作为年龄的函数被编入目录。然后我们将继续研究 已经开始尝试识别表型变化 在前列腺增生症中观察到的每一种病变的特征。我们有 最近描述了一种新的方法，允许作为异种移植生长 大多数前列腺癌(PCa)。我们观察到，有了这个新的 异种移植的方法，在一些人的异种移植物中形成上皮性肿瘤 来自无前列腺癌患者的前列腺增生症样本；该系统将用于 确定哪些病变能够作为异种移植生长，并 定义增长分数、增长潜力和表达差异的能力 有无免疫缺陷小鼠的受精标志物 外源提供的生长因子。在初步实验中，我们有 在一些BPH样本中发现EGF-R的多条消息(类似于 在A431恶性细胞系中描述的那些)，过表达 转化生长因子-α，以及附近基因缺失的证据 1OQ24。我们将调查这些标记和其他标记以确定 将这些实体彼此区分开来并更好地 了解这些实体的生物学潜力。

Benign prostatic hyperplasia (BPH) is a very common disease in the aging male. Both BPH and chronic prostatitis are pathologically imprecisely defined. Prostatitis, as detailed in the body of this proposal, has been described by one prominent urologist as a 'wastebasket of clinical ignorance.' Both of these disease states (a) are more common among diabetics and (b) involve changes in the prostate that are, at the level of the tissue, both multiple and diverse. Attempts to understand the pathogenesis, biochemistry, molecular biology, endocrinology, and immunology of these lesions will be very frustrating until precise definitions of the many diverse entities in the 'wastebasket' can be defined. This task will be approached with the morphometric quantification of the anatomical and pathological structures that are observed in men starting with men in their early twenties. Alternating serial sections of whole prostates at intervals of 0.5 cm will be processed in paraffin and methacrylate. With corrections for differences in tissue shrinkage with these two methods, a computer-assisted three-dimensional image of each prostate will be constructed. The use of methacrylate allows superior in situ hybridization and the demonstration of most enzymes and antigens. The use of paraffin allows the recovery of nuclei that are satisfactory for use with the polymerase chain reaction and for flow cytometry. The frequency of each recognizable lesion will be catalogued as a function of age. We shall then continue a study already begun in an attempt to identify phenotypic changes that are characteristic of each of the lesions that are observed in BPH. We have recently describe a new method that permits the growth as xenografts of most prostatic carcinomas (PCA). We have observed that, with this new method for xenografting, epithelial tumors form in the xenografts of some samples of BPH from patients without PCA; this system will be used to determine which lesions are capable of growing as xenografts and to define growth fractions, growth potentials, and ability to express dif- ferentiation markers in immunologically deficient mice with and without exogenously supplied growth factors. In preliminary experiments, we have found in some samples of BPH multiple messages for EGF-R (similar to those described in the A431 line of malignant cells), overexpression of TGF-alpha, and evidence of the deletion of genes in the vicinity of 1Oq24. We shall investigate these and other markers to define differences that distinguish these entities from each other and to better understand the biological potentials of these entities.