60 HZ MAGNETIC FIELD-CLOCKS & REPRODUCTIVE DEVELOPMENT

描述(改编自申请人的摘要)：提案将 确定60赫兹磁场(MF)暴露是否具有生物性 对生殖发展不利的影响。这个 实验计划，部分将定义MF暴露条件， 可能会影响与中央服务器相关的各种终端 性成熟的神经系统控制力。细胞和分子 标记物将被用来检验关于MF的机制的假设 暴露可能会影响生殖发育。在《荒野猎人》中 仓鼠，青春期的过程可以被诱导或阻止 环境光周期。在漫长的日子里，是 青春期的启动(15-25天龄)与快速 性腺发育，促性腺激素分泌和 内侧单极促性腺激素释放激素神经元数量增加 大脑的视前区和斜角带区。这些指数中的每个 通过暴露在短日照下维持青春期前的水平，这是一种影响 这是由松果体褪黑激素的昼夜节律调节的 制作。在不同物种中暴露于MF，包括 丛林仓鼠，已被发现抑制夜间上升 松果体褪黑激素的生产。如果暴露在MF中的影响 褪黑激素的节律具有生理意义，然后 传递光周期信息的机制将导致 生殖系统对短白天的不适当反应。 实验计划集中在MF的作用机制上。 褪黑素与生殖发育。目标是确定 MF暴露是否通过内源性生物效应影响守时 在松果体上产生褪黑激素节律的时钟。 脑和松果体对甲醛暴露的分子反应研究 腺体包括转录激活，如表达所示 C-fos和NGFIA等即刻早期基因以及与时钟相关的基因 Per基因的精氨酸加压素同源物的消息。进一步 MF暴露对GnRH的直接细胞作用的考虑 在青春期开始和个体发育过程中的神经系统导致 促性腺激素释放激素细胞的数量、分布、形态、迁移和 在发展的关键阶段进行预测。研究方法 使用已建立的动物模型系统来测试关于 MF暴露对生物计时的影响及其神经机制 它控制着生殖发育。

DESCRIPTION (Adapted from applicant's abstract):The proposal will determine whether 60-Hz magnetic field (MF) exposures have biological effects that are detrimental to reproductive development. The experimental plan, in part, will define the MF exposure conditions that may influence a variety of endpoints that are relevant to the central nervous system control of sexual maturation. Cellular and molecular markers will be used to test a hypothesis about the mechanism by which MF exposures may influence reproductive development. In the Djungarian hamster, the process of puberty can be induced or arrested by environmental photoperiod. In long days, a critical period for initiation of puberty (15-25 days of age) is associated with rapid gonadal development, a dramatic elevation in gonadotropin secretion and increased numbers of morphologically unipolar GnRH neurons in the medial preoptic and diagonal band regions of the brain. Each of these indices are maintained a prepubertal levels by exposure to short days, an effect that is mediated by the circadian rhythm in pineal gland melatonin production. MF exposure, in a diverse number of species, including the Djungarian hamster, has been found to suppress the night-time rise in pineal melatonin production. If the effect of MF exposure on the melatonin rhythm is of physiological significance, then a disruption in the mechanism that conveys photoperiod information will result in an inappropriate response to short days by the reproductive system. Experiments are planned to focus on the mechanism of MF effects on melatonin and reproductive development. The objective is to determine whether MF exposure affects timekeeping by the endogenous biological clock that generates the melatonin rhythm or on the pineal itself. Studies of the molecular responses to MF exposure in the brain and pineal gland include transcriptional activation, as indicated by the expression of the immediate early genes, c-fos and NGFIA, as well as clock-related messages for arginine vasopressin homologues of the per gene. Further consideration of a direct cellular action of MF exposure on the GnRH neuron system at the onset of puberty and during its ontogeny leads to studies of GnRH cell number, distribution, morphology, migration, and projections during critical stages of development. The research approach uses an established animal model system to test hypotheses about the effects of MF exposure on biological timekeeping and the neural mechanism that controls reproductive development.