DEVELOPMENT AND MAINTENANCE OF LENS TRANSPARENCY

透明度是基本的结构特征， 将晶状体细胞与所有其他哺乳动物细胞区分开来。在镜头中 单元格，当空间波动时建立透明度 细胞质密度相对于波长变小 光，最大限度地减少光散射。空间的大小 波动取决于细胞质之间的分子相互作用 晶状体细胞的成分。这些分子的变化 相互作用可能会造成透明度的丧失和形成 白内障。 本申请是关于发展的研究的继续 并保持镜头的透明度。之前的提案开始了 晶状体透明度发展的研究，鉴定为可逆的 晶状体混浊的分期和发现的全新试剂 用于预防辐射所致大鼠浑浊。这个 本申请建议继续研究开发 透明度，以表征负责的分子相互作用 体内正常发育和维持晶状体透明度。 并在几种白内障模型上测试新试剂： 半乳糖型、亚硒酸盐、链脲佐菌素和R.C.S.(皇家学院 (外科大鼠) 在拟议的研究计划中，研究将(1)评估 晶状体胞质空间波动的定量研究 胚胎晶状体细胞透明的正常发育； 描述与正常有关的分子相互作用 透明晶状体细胞的空间波动，以及(3)证明 新型相分离缓蚀剂的药效研究 活体白内障的预防。 工作假设是对空间的直接分析 晶状体细胞在发育过程中的波动将导致 对调节透明的分子相互作用的理解 细胞结构。

Transparency is the fundamental structural feature that distinguishes lens cells from all other mammalian cells. In lens cells, transparency is established when spatial fluctuations in cytoplasmic density become small relative to the wavelength of light, which minimizes light scattering. The size of the spatial fluctuations depends on molecular interactions between cytoplasmic constituents of lens cells. Changes in these molecular interactions can produce loss of transparency and formation of cataracts. The present application is a continuation of studies on development and maintenance of lens transparency. The previous proposal began studies on development of lens transparency, identified reversible stages of lens opacification and discovered completely new reagents for preventing opacification in rats exposed to Irradiation. The present application proposes continued research on development of transparency to characterize molecular interactions responsible for normal development and maintenance of lens transparency in vivo. and to test the new reagents on several cataract models: galactosemic, selenite, streptozotocin and R.C.S. (Royal College of Surgeons rat) In the proposed research plan, the studies will (1) evaluate quantitatively the spatial fluctuations in lens cytoplasm during normal development of transparency in embryonic lens cells; (2) characterize the molecular interactions responsible for normal spatial fluctuations in transparent lens cells, and (3) demonstrate the effectiveness of new phase separation inhibitors on the prevention of cataracts in vivo. The working hypothesis is that direct analysis of the spatial fluctuations in lens cells during development will lead to an understanding of molecular interactions that regulate transparent cell structure.