BIOCHEMICAL RESPONSES OF HUMAN CILIARY EPITHELIAL CELLS

关于房水分泌和房水调节的知识 人类眼睛中的产物与理解和 青光眼的治疗。房水是由纤毛分泌的。 上皮细胞及其组成在很大程度上取决于 血浆蛋白质和电子的主动转运机制 在这些牢房里。房水的形成速度是 由交感(肾上腺素)系统调制(Neufeld，A.H.， 1984年)。 纤毛组织培养系统的研制 上皮(CE)细胞，即无色素(NPE)和 色素(PE)上皮很重要，因为它允许 这些细胞的细胞和分子功能的体外研究 这些都很难在体内进行。 这项提议的目的是研制出针对这两种病毒的抗体。 膜结合的表面蛋白和内部蛋白，它们是 利用杂交瘤技术检测NPE和PE细胞的特异性标志物 技术这些抗体将作为探针用于研究 这些细胞在培养中的分化特性。此外, 它将允许分离(即荧光激活的细胞 基于其潜力的NPE和PE细胞的分类器) 免疫学上的差异。 这些抗体将被用来促进对 NPE和PE细胞的分泌功能和转运。 将通过开发一种抗体来研究分泌功能 CE在培养中分泌76 KDa蛋白。离子传输将是 免疫印迹法检测(Na+，K+)-ATPase活性 通过这种酶的阿尔法亚单位的抗体。钠泵 运输活动将由哇巴因敏感的86Rb+评估 领悟。 此外，外部信号的转导机制 转化为细胞内事件(即蛋白质磷酸化) 学习。

Knowledge of aqueous humor secretion and regulation of aqueous production in the human eye is relevant to the understanding and treatment of glaucoma. Aqueous humor is secreted by the ciliary epithelium and its composition is largely dependent on the mechanism of active transport of plasma proteins an electrolites in these cells. The rate of formation of aqueous humor is modulated by the sympathetic (adrenergic) system (Neufeld, A.H., 1984). The development of a tissue culture system for the ciliary epithelial (CE) cells, namely the nonpigmented (NPE) and pigmented (PE) epithelia is important because it permits the in vitro study of the cellular and molecular functions of these cells which are very difficult to carry out in vivo. The aim of this proposal is to develop antibodies to both membrane-bound surface proteins and internal proteins which are specific markers for NPE and PE cells using the hybridoma technology. These antibodies will serve as probes to investigate the differentiated properties of these cells in culture. In addition, it will allow for the isolation (i.e. fluorescence activated cell sorter) of NPE and PE cells based on their potential immunological differences. These antibodies will be used to facilitate the understanding of the secretory function and transport of NPE and PE cells. Secretory function will be studied by developing antibodies to a 76KDa protein secreted by CE in culture. Ion transport will be studied by immunobloting analysis of (Na+, K+)-ATPase activity by antibodies to the alpha subunit of this enzyme. Sodium pump transport activity will assessed by ouabain-sensitive 86Rb+ uptake. In addition, the mechanism of transduction by external signals into intracellular events (i.e., protein phosphorylation) will be studied.