THE STUDY OF CELL JUNCTIONS IN THE OCULAR LENS

我们建议研究紧密连接，缝隙连接和方形阵列， 青蛙、小鼠和兔子的眼晶状体。 拟议研究的长期目标是获得更好的 认识中的产状、构造特征和作用 结复合体和透镜中的方形阵列。 本研究计划的具体目标如下：（1）研究 透镜中紧密连接的存在和结构特征 兔、小鼠和青蛙的上皮细胞。 (2)确定渗透性 以及正常情况下紧密连接的作用。 (3)探讨 无钙培养基对紧密连接结构的影响， 青蛙透镜的渗透性。 (4)分析结构差异 蛙透镜上皮细胞和纤维细胞之间缝隙连接的研究 解偶联剂，如缺氧和低pH值。（5）检查可能的变化 埃默里小鼠白内障的缝隙连接结构和分布 在衰老和白内障形成过程中。 (6)调查事件， 结构组织和方形阵列在透镜中的作用， 兔子和老鼠 (7)研究方阵的分布变化， Emory小鼠白内障纤维细胞膜表面嵴纹的研究 在衰老和白内障发生过程中：超微结构分析。 （八） 确定主要膜蛋白（MP26和MP22）的可能变化 与方形阵列和表面脊的分布变化有关 埃默里小鼠衰老和白内障发生期间的白内障：A 生化分析 所涉及的方法将是扫描和透射电子显微镜， 冷冻断裂TEM，示踪技术，细胞化学，蛋白质分离， SDS-聚丙烯酰胺凝胶电泳等

We propose to study the tight junctions, gap junctions and square arrays in the ocular lenses of frog, mouse and rabbit. The long-term objectives of the proposed research are to gain a better understanding in the occurrence, structural characteristics and the role of the junctional complexes and of the square arrays in the lens. The specific aims of the research plan are as follows: (1) Study the occurrence and structural characteristics of tight junctions in lens epithelia of rabbit, mouse and frog. (2) Determine permeability properties and the role of tight junctions under normal conditions. (3) Investigate the effect of calcium-free medium on the tight junctional structure and permeability of the frog lens. (4) Analyze the structural difference between gap junctions of epithelium and fiber cells of frog lens using uncoupling agents such as anoxia and low pH. (5) Examine possible changes of gap junctional structure and distribution in the Emory mouse cataract during aging and cataractogenesis. (6) Investigate the occurrence, structural organization and the role of square arrays in the lenses of rabbit and mouse. (7) Study the distribution changes of square arrays and surface ridge patterns of fiber cell membrane in the Emory mouse cataract during aging and cataractogenesis: An ultrastructural analysis. (8) Determine the possible alteration of major membrane proteins (MP26 & MP22) as related to the distribution changes of square arrays and surface ridges in the Emory mouse cataract during aging and cataractogenesis: A biochemical analysis. Methodology involved will be scanning and transmission electron microscopy, freeze-fracture TEM, tracer techniques, cytochemistry, protein isolation, SDS-Polyacrylamide Gel Electrophoresis, etc.