DEVELOPMENT AND MAINTENANCE OF TRANSPARENCY IN LENSES

透明度是基本的结构特征， 将透镜细胞与所有其他哺乳动物细胞区分开来。 在透镜内 像元中的空间波动时， 细胞质密度相对于波长变小， 光，最大限度地减少光散射。 空间的大小 波动取决于细胞质之间的分子相互作用 透镜细胞的组成部分。 这些分子的变化 相互作用会导致透明度的丧失， 白内障 本申请是关于发展的研究的继续。 和保持透镜的透明度。 上一个提案开始于 透镜透明度发展研究，确定可逆 透镜浑浊化的各个阶段，并发现了全新的试剂 用于防止暴露于辐射的大鼠的混浊。 的 本申请建议继续研究开发 透明度，以表征负责 体内透镜透明度的正常发育和维持。 并在几种白内障模型上测试新试剂： 半乳糖、亚硒酸盐、链脲佐菌素和R.C.S.（皇家学院 外科医生大鼠） 在拟议的研究计划中，研究将（1）评估 定量地测量了在晶状体形成过程中透镜细胞质中的空间波动。 胚胎透镜细胞透明性的正常发育;（2） 表征负责正常的分子相互作用 透明透镜单元中的空间波动，以及（3）证明 新的相分离抑制剂的有效性 在体内预防白内障。 工作假设是，直接分析的空间 透镜细胞在发育过程中的波动将导致 了解分子间的相互作用， 细胞结构

Transparency is the fundamental structural feature that distinguishes lens cells from all other mammalian cells. In lens cells, transparency is established when spatial fluctuations in cytoplasmic density become small relative to the wavelength of light, which minimizes light scattering. The size of the spatial fluctuations depends on molecular interactions between cytoplasmic constituents of lens cells. Changes in these molecular interactions can produce loss of transparency and formation of cataracts. The present application is a continuation of studies on development and maintenance of lens transparency. The previous proposal began studies on development of lens transparency, identified reversible stages of lens opacification and discovered completely new reagents for preventing opacification in rats exposed to Irradiation. The present application proposes continued research on development of transparency to characterize molecular interactions responsible for normal development and maintenance of lens transparency in vivo. and to test the new reagents on several cataract models: galactosemic, selenite, streptozotocin and R.C.S. (Royal College of Surgeons rat) In the proposed research plan, the studies will (1) evaluate quantitatively the spatial fluctuations in lens cytoplasm during normal development of transparency in embryonic lens cells; (2) characterize the molecular interactions responsible for normal spatial fluctuations in transparent lens cells, and (3) demonstrate the effectiveness of new phase separation inhibitors on the prevention of cataracts in vivo. The working hypothesis is that direct analysis of the spatial fluctuations in lens cells during development will lead to an understanding of molecular interactions that regulate transparent cell structure.