THE STUDY OF CELL JUNCTIONS IN THE OCULAR LENS

我们建议研究密结、缝隙结和正方形阵列 青蛙、小鼠和兔的晶状体。 拟议研究的长期目标是获得更好的 认识中的发生、结构特征和作用 晶状体中的连接复合体和正方形阵列。 研究计划的具体目标如下：(1)研究 晶状体中紧密连接的产生及其结构特征 兔、小鼠和蛙的上皮细胞。(2)确定渗透率特性 以及正常情况下紧密连接的作用。(三)调查 无钙介质对紧密连接结构和细胞周期的影响 青蛙晶状体的透过率。(4)结构差异分析 青蛙晶状体上皮细胞与纤维细胞间缝隙连接的研究 解偶联剂，如缺氧和低pH。(5)研究可能发生的变化 Emory小鼠白内障缝隙连接结构及分布的研究 在衰老和白内障形成过程中。(六)调查发生情况， 结构组织和正方形阵列在透镜中的作用 兔子和老鼠。(7)研究正方形阵列的分布变化和 Emory小鼠白内障中纤维细胞膜的表面纹饰 衰老与白内障发生过程中的超微结构分析(8) 确定主要膜蛋白(MP26和MP22)可能的变化 与方阵和表面脊的分布变化有关 在Emory小鼠白内障的衰老和白内障发生过程中：A 生化分析。 所涉及的方法将是扫描和透射电子显微镜， 冷冻断裂电子显微镜，示踪技术，细胞化学，蛋白质分离， SDS-聚丙烯酰胺凝胶电泳法等。

We propose to study the tight junctions, gap junctions and square arrays in the ocular lenses of frog, mouse and rabbit. The long-term objectives of the proposed research are to gain a better understanding in the occurrence, structural characteristics and the role of the junctional complexes and of the square arrays in the lens. The specific aims of the research plan are as follows: (1) Study the occurrence and structural characteristics of tight junctions in lens epithelia of rabbit, mouse and frog. (2) Determine permeability properties and the role of tight junctions under normal conditions. (3) Investigate the effect of calcium-free medium on the tight junctional structure and permeability of the frog lens. (4) Analyze the structural difference between gap junctions of epithelium and fiber cells of frog lens using uncoupling agents such as anoxia and low pH. (5) Examine possible changes of gap junctional structure and distribution in the Emory mouse cataract during aging and cataractogenesis. (6) Investigate the occurrence, structural organization and the role of square arrays in the lenses of rabbit and mouse. (7) Study the distribution changes of square arrays and surface ridge patterns of fiber cell membrane in the Emory mouse cataract during aging and cataractogenesis: An ultrastructural analysis. (8) Determine the possible alteration of major membrane proteins (MP26 & MP22) as related to the distribution changes of square arrays and surface ridges in the Emory mouse cataract during aging and cataractogenesis: A biochemical analysis. Methodology involved will be scanning and transmission electron microscopy, freeze-fracture TEM, tracer techniques, cytochemistry, protein isolation, SDS-Polyacrylamide Gel Electrophoresis, etc.