VITAMIN A AND CONJUNCTIVAL TRANSDIFFERENTIATION

异常上皮分化是两种主要的 眼表疾病的分类。 第一， 结膜上皮呈鳞状化生，杯状 细胞丢失、细胞增大和角化，伴随 泪膜不稳定和角膜变化， 视野 在第二种情况下，角膜上皮被替换为 结膜上皮（结膜化）， 伴随着表面的不规则和血管化， 视力下降。 我们计划进一步的实验来阐明类维生素A在 调节上皮分化，目的是更好地 管理这些类别的眼表疾病。 我们计划 目的：（1）研究结膜上皮细胞对结膜上皮细胞的调节作用 分化的维甲酸在体外，使用我们的单细胞克隆 增长模式 表型表达的每个菌落来源于 上皮分化的各个阶段将通过以下步骤确定： 使用细胞类型的生物化学和免疫组织化学分析 特异性单克隆抗体（MNAB）。 (2)探讨 粘蛋白与顶膜间的分子相互作用 蛋白质使用MNABs方法。 约束力研究将 进行了原位和体外实验。 (3)研究利用 光凝法阻断诱导角膜新生血管 结膜样上皮的转分化 角膜变成角膜样上皮。 (4)探讨是否可能以 角膜缘上皮细胞在角膜表面重建中的作用 结膜迁移的屏障。

Abnormal epithelial differentiation is the hallmark of two major categories of ocular surface disorders. In the first, the conjunctival epithelium exhibits squamous metaplasia, with goblet cell loss, cell enlargement and keratinization, with accompanying tear film instability and corneal changes that result in reduced vision. In the second, the corneal epithelium is replaced by conjunctival epithelium ("conjunctivalization"), with accompanying surface irregularity and vascularization that result in reduced vision. We plan further experiments to elucidate the role of retinoids in modulating epithelial differentiation, with the goal of better managing these categories of ocular surface disorders. We plan to: (1) Study the modulation of conjunctival epithelial differentiation by retinoids in vitro, using our single-cell clonal growth model. Phenotypic expression of each colony derived from various stages of epithelial differentiation will be determined by biochemical and immunohistochemical analyses using cell-type specific monoclonal antibodies (MNABs). (2) Investigate molecular interactions between mucin and apical membrane proteins using MNABs approaches. The binding studies will be conducted with in situ and in vitro. (3) Study the use of photothrombosis for occlusion of corneal new vessels in inducing transdifferentiation of a conjunctiva-like epithelium on the cornea into a cornea-like epithelium. (4) Explore the potential of limbal epithelial cells in corneal re-surfacing and their role as a barrier to conjunctival migration.