ANESTHETIC ACTION AND VIRUS REPLICATION

这项拟议的研究将检验吸入麻醉剂的作用。 关于病毒在体内和体外的生物学和生物化学。 麻疹病毒、单纯疱疹病毒和脊髓灰质炎病毒将在组织中生长 培养并暴露于各种吸入麻醉剂。这个机制是通过 将对哪些麻醉剂影响病毒复制进行调查。这 将通过分析病毒核酸和蛋白质合成来完成 在受感染的培养物暴露于麻醉剂期间作为病毒组装。 由于临床麻醉被体内高压逆转，任何 提出的分子水平上的麻醉理论必须考虑 这一观察结果。我们已经确定了一种使用麻疹病毒膜的系统 被吸入麻醉剂抑制的蛋白质。这 抑制在高压条件下被逆转，因此，这些 研究将有助于获得有关药物作用的分子机制的知识 麻醉剂。在体外获得的结果将与 活体研究和涉及到的患者的检查 呼吸道感染，正在接受麻醉和手术治疗 鼓膜切开鼓膜造孔置管术。

The proposed research will examine the actions of inhalational anesthetics on the biology and biochemistry on viruses both in vivo and in vitro. Measles virus, herpes simplex virus, and poliovirus will be grown in tissue culture and exposed to various inhalational anesthetics. The mechanism by which the anesthetics affect virus replication will be investigated. This will be done by analyzing virus nucleic acid and protein synthesis as well as virus assembly during exposure of the infected cultures to anesthetics. Since clinical anesthesia is reversed by hyperbaric pressure in vivo, any proposed theory of anesthesia at the molecular level must take into account this observation. We have identified a system using measles virus membrane proteins which are inhibited by inhalational anesthetic exposure. This inhibition is reversed under hyperbaric conditions, therefore, these studies will help gain knowledge into the molecular mechanism of action of anesthetic agents. The results obtained in vitro will be correlated with the in vivo study and involve the examination of patients who have upper respiratory tract infections and are undergoing anesthesia and surgery for myringotomy and tympanostomy tube placement.