GENETIC BASIS OF SELECTION IN NATURAL POPULATIONS

使用适当的遗传程序生产等基因菌株 大肠杆菌和适用于阳性和阴性对照的培养基时，我们有 成功地分析了4个等位基因决定的选择效应 6-磷酸葡萄糖酸脱氢酶的自然产生的等位酶。 这些等位基因在K-12遗传背景中是选择性中性的。我们现在 建议对与其他一些基因相关的基因座进行类似的研究 等位基因多态性，包括酒精脱氢酶、谷氨酸草酰乙酸酯 转移酶，葡萄糖-6-磷酸脱氢酶，磷酸葡萄糖异构酶， 甘油醛-3-磷酸脱氢酶、多肽酶、甘露糖磷酸 异构酶、异柠檬酸脱氢酶和磷酸葡萄糖变位酶。可能的多个 此外，还将研究某些生化指标对体能的影响。 发现与选择有关的等位酶的特征。这些 研究与选择性中立性假设有关，因为它与 等位基因。

Using appropriate genetic procedures for the production of isogenic strains of E. coli and appropriate media for positive and negative controls, we have successfully analyzed the selective effects of four alleles determining naturally occurring allozymes of the enzyme 6-phosphogluconate dehydrogenase. The alleles are selectively neutral in the K-12 genetic background. We now propose to carry out similar studies of loci associated with a number of other allozyme polymorphisms, including alcohol dehydrogenase, glutamic oxaloacetate transferase, glucose-6-phosphate dehydrogenase, phosphoglucose isomerase, glyceraldehyde-3-phosphate dehydrogenase, peptidase, mannose phosphate isomerase, isocitrate dehydrogenase, and phosphoglucomutase. Possible multiple locus effects on fitness will also be examined, as will certain biochemical characteristics of allozymes found to be associated with selection. These studies bear on the hypothesis of selective neutrality as it pertains to allozymes.