TIMING OF MAMMALIAN CELL CYCLE EVENTS

我们建议研究细胞计时的动力学，使用 同步和非同步动物细胞培养。假设 短周期的量子钟负责细胞行为的特征 例如温度补偿和相位响应性将被测试 使用数字视频显微镜、图像处理和流程 微量荧光法。认为时钟表现为极限环的概念 我们还将探索振荡器。细胞大小作为边界的作用 振子表达式中的条件以及这一点的可能性 互动导致消极的母女关系和积极的姐妹关系 将测试细胞生成时间的相关性。细胞夹带 除以生长因素的组合和温度变化将 尝试使用已知的细胞周期时相响应行为来响应这些介质 作为背景。携带细胞分裂基因在体内的表达 将使用中胚菌来探索生长因子水平的变化 极光。

We propose to examine the dynamics of cellular timekeeping using synchronous and non-synchronous animal cell cultures. The hypothesis that a short period, quantal clock is responsible for features of cell behavior such as temperature compensation and phase responsiveness will be tested using digital video microscopy, image processing and flow microfluorometry. The notion that the clock behaves as a limit cycle oscillator will also be explored. The role of cell size as a boundary condition in the expression of the oscillator and the possibility that this interaction leads to negative mother-daughter and positive sister-sister correlations in cell generation times will be tested. Entrainment of cell division by combinations of growth factors and by temperature shifts will be attempted using known cell cycle phase response behavior to these agents as a background. In vivo expression of entrainment of cell division by changing levels of growth factor will be explored using Mesocricetus auratus.