Exploring the chopping board microbiome

许多食源性感染始于家庭，通常是由于不卫生，切菜板为生食交叉污染提供了机会。通过对菜板进行微生物分析，包括调查发现的微生物的抗菌素耐药性特征，我们将解决“确保食品安全和标准”优先领域的两个方面：食品标准局如何更好地了解和减少食源性病原体的影响？食品标准局如何改进有关抗菌素耐药性（AMR）和食品的证据基础？我们将结合贡献、合作和共同创造的公民科学方法，利用我们的生物科学本科生作为大使，前往难以到达的社区。大使和公民将参与议定书和材料的设计，以及最后的传播产出，包括面向社区的教育材料和学术论文。我们有六项一揽子工作，使我们能够实现我们的目标。WP1 -招募公民科学家。我们的生物科学学生承担微生物实践工作作为其计划的核心部分。通过我们的学生社区，我们将招募约150-200名大使，他们将有特权进入他们的家庭和社区，并提供快速的样本收集，因为他们将定期前往校园。参加者将在大使的协助下收集样本。WP2 -共同创建采样方法。我们将与一部分大使和他们的家庭共同制定最终的抽样方法。这些将被设计为可靠和可重复的，同时提供可靠的科学结果，并为最终用户所接受。我们将共同创建教学材料，以确保它是有效的和适合的目的。WP3 -砧板取样。大使们将收集抽样工具包，并让他们的家庭参与抽样。除此之外，他们还会记录相关的人口统计信息，以及与砧板使用和行为信息有关的关键参数。WP4 -样本分析。这将由我们的项目经理或参与者自己在“开放会议”中进行，他们可以访问实验室并自己进行微生物测试。WP6 -传播。我们将利用社会媒体，包括市民的“收购”，在整个过程中宣传这个项目。学术团队和我们的大使将为我们的公民和更广泛的社区举办项目结束展示会，展示项目及其成果。最终，我们希望为每个社区开发出砧板使用的最佳实践指南。利用我们的科学发现，我们的大使和公民将与学术团队共同创作专门针对我们的社区团体分发的教育材料，以促进行为改变。我们将公布该项目的微生物学和公民科学成果，我们的大使和公民参与撰写手稿，并酌情命名为作者。WP7 -监测和评估。我们将根据参与的次数（大使和参与者分别记录）量化项目的成功程度。我们的项目经理将重复关键结果，以确保我们发现的科学可重复性。我们将通过电子调查和个人访谈来评估社会效益。对于我们的参与者，我们将评估食品卫生知识和行为的变化，对于我们的大使，我们将评估他们公民科学知识的发展。

Many foodborne infections begin in the home, often through poor hygiene where chopping boards provide an opportunity for raw foods to cross-contaminate. By undertaking microbiological analysis of chopping boards, including investigating the antimicrobial resistance profiles of the organisms found, we will address two of the areas of the priority area "assuring food safety and standards":How can the FSA better understand and reduce the impact of foodborne pathogens?How can the FSA improve the evidence base concerning antimicrobial resistance (AMR) and food?We will use a combination of contributary, collaborative and co-creation citizen science approaches whereby we will use our undergraduate Bioscience students as ambassadors to hard-to-reach communities. Ambassadors and citizens will be involved in the design of protocols and materials, as well as the final dissemination outputs including educational materials for communities and academic papers. We have six work packages that allow us to achieve our objectives.WP1 - Recruitment of citizen scientists. Our Biosciences students undertake microbiological practical work as a core part of their programme. Through our student community, we will recruit ~150-200 ambassadors who will give us privileged access to their homes and communities, as well as providing rapid sample collection as they will be travelling to campus regularly. Samples will be collected by our participants using the ambassadors as facilitators.WP2 - Co-creation of sampling methods. With a subset of our ambassadors and their households, we will co-create our final sampling methods. These will be designed to be robust and reproducible whilst providing sound scientific outcomes and being acceptable to the end users. We will co-create the instructional material to ensure it is effective and fit-for-purpose.WP3 - Chopping board sampling. Ambassadors will collect sampling kits and engage their households with sampling. Alongside this, they will record the relevant demographic information as well as key parameters relating to chopping board use and behavioural information.WP4 - Sample analysis. This will be conducted either by our project manager or by the participants themselves at "open sessions" where they can visit the laboratory and undertake the microbiological testing themselves.WP6 - Dissemination. We will use social media, including "takeovers" by our citizens to publicise the project throughout. The academic team and our ambassadors will run an end-of-project showcase for our citizens and the wider community to present the project and its outcomes. Ultimately, we want to develop best practice guidance for chopping board use for each community. Using our scientific findings, our ambassadors and citizens will co-create, with the academic team, educational materials specifically targeted to our community groups for distribution to promote behavioural change. We will publish the microbiological and citizen science outcomes of the project with our ambassadors and citizens involved in writing the manuscripts and named as authors as appropriate.WP7 - Monitoring and evaluation. We will quantify project success in terms of number of engagements (ambassadors and participants recorded separately). Our project manager will replicate key results to ensure scientific reproducibility of our findings. We will evaluate the societal benefits through electronic surveys and individual interviews. For our participants we will assess changes in food hygiene knowledge and behaviour and, for our ambassadors, development in their knowledge of citizen science.