ACCURACY AND EFFICIENCY OF PROTEIN BIOSYNTHESIS

蛋白质合成是所有细胞的基本活动。 已知的是 对细胞的能量和物质资源提出了非常大的需求。 很可能，即使是这项活动中的小缺陷也会非常严重。 对细胞功能有害。 除了它对人类的直接重要性之外， 细胞，充分了解蛋白质合成将是至关重要的，在不久的将来， 未来的廉价蛋白质药物的制备。 这项研究的长期目标是了解细胞是如何 进行蛋白质合成的成分相互作用， 准确有效地执行活动。 一种具体 在这个项目期间的目的是显示如何mRNA的结构， tRNA影响这些特性。 另一个目的是阐明机制 核糖体通过其选择aa-tRNA。 将使用动力学技术进行调查， 了解参与蛋白质合成的分子如何相互作用， 体外 使用快速混合装置和高度纯化的组分， 将确定产品的生产率。 这些利率将使 关于蛋白质的各种成分 合成装置相互作用。 根据对这些物质浓度的了解， 细胞中的这些成分，将有可能重建事件， 发生在细胞内部，无法直接观察到。

Protein synthesis is a fundamental activity of all cells. It is known to place very large demands on the energy and material resources of the cell. It is likely that even minor defects in this activity would be very detrimental to cellular functions. Besides its direct importance to human cells, a full understanding of protein synthesis will be vital in the near future for the preparation of inexpensive protein pharmaceuticals. The long-tem goal of this research is to understand how the cellular components that conduct protein synthesis interact to ensure that this activity is carried out accurately and efficiently. One of the specific aims during this project period is to show how the structure of mRNA and tRNA affect these properties. Another aim is to elucidate the mechanism through which aa-tRNAs are selected by the ribosome. The investigations are to be conducted using kinetic techniques to understand how the molecules involved in protein synthesis interact in vitro. Using a rapid mixing apparatus and highly purified components, the rate at which products are made will be determined. These rates will give important information about the way the various components of the protein synthesis apparatus interact. From a knowledge of the concentrations of these components in the cell it will be possible to reconstruct events that occur inside the cell that are not accessible to direct observation.