ELECTRON TRANSFER DYNAMICS IN HEME PROTEINS

这项提案的长期目标是对 伴随着活性中心的电子转移的结构动力学 血红素蛋白质和蛋白质复合体。这将使我们能够确定 这一重要生理过程的分子机制是 在活细胞的呼吸过程中被调节的。我们将聘用 跟踪电子转移动力学的时间分辨光学光谱仪 在适当的时间尺度上。线粒体电子的组成 运输链，特别是细胞色素c氧化酶和bc1复合体， 将使用时间分辨共振拉曼技术进行研究 已经在我们的实验室里买到了。几类新的光活性物质 分子将被用来启动蛋白质系统的电子流动。 处于特定的氧化还原或反应状态。这 技术将使我们能够分离和表征 蛋白质构象动力学控制的速度和特异度 多组分络合物中的电子流动。

The long-term goal of this proposal is the characterization of the structural dynamics that accompany electron transfer at the active sites of heme proteins and protein complexes. This will allow us to determine the molecular mechanisms by which this important physiological process is regulated during respiration in living cells. We will employ time-resolved optical spectroscopies to follow electron transfer dynamics on appropriate timescales. Components of the mitochrondrial electron transport chain, specifically cytochrome c oxidase and bc1 complexes, will be investigated using time-resolved resonance Raman techniques already available in our laboratory. Several new classes of photoactive molecules will be used to initiate electron flow with protein systems that have been poised in specific redox or reactive states. This technique will permit us to isolate and characterize the extent to which protein conformational dynamics control the rate and specificity of electron flow in multi-component complexes.