REDUCTIVE CLEAVAGE METHOD FOR GLYCAN STRUCTURAL ANALYSIS

复杂碳水化合物结构的测定是一个重要的研究领域。 许多生物医学研究领域都存在重要问题， 参与细胞识别过程， 作为结构材料，它们的存在作为抗原 肿瘤、细菌、真菌和病毒中的决定簇。 因为 它们的结构复杂，没有单一的方法来建立 其结构是可用的。 无数的化学方法 目前可用的是非常费力和模糊的结果， 经常获得。 因此，我们开始了一项计划， 用于复杂聚糖的结构表征的方法， 将克服与当前的 方法论。 构成本建议基础的结构方法是 被称为“还原裂解法”，因为它的突出 特征是所有糖苷的区域特异性还原裂解 完全甲基化多糖中的键。 该方法 然而，与“甲基化分析”基本上不同， 因为1）多糖中的所有键合位置和环形式 同时建立，2）完全转换 甲基化多糖到适于分离的衍生物 和分析是在一个过去的工作完成，我们觉得， 还原裂解法有可能作为一种通用的 全配合物的结构表征方法 碳水化合物，包括确定 组成单体、它们的环形式、它们的位置 连接、它们的异头构型和它们的序列。 设计实验来检验这种方法的可行性 这是这项提议的基础。 本文提出的研究 因此，它的目标是整合还原性 复杂碳水化合物的简化裂解法 结构分析

The determination of structure of complex carbohydrates is an important problem in many areas of biomedical investigation because of their involvement in cellular recognition processes, their role as structural materials, and their presence as antigenic determinants in tumors, bacteria, fungi, and viruses. Because of their structural complexity, no single method for establishing their structure is available. The myriad of chemical methodologies currently available are very laborious and ambiguous results are often obtained. We therefore began a program to develop a new method for the structural characterization of complex glycans that would overcome the difficulties associated with current methodologies. The structural method that forms the basis of this proposal is referred to as the "Reductive Cleavage Method" because its salient feature is the regiospecific reductive cleavage of all glycosidic bonds in a fully methylated polysaccharide. This method is substantially different than "methylation analysis", however, because 1) all linkage positions and ring forms in a polysaccharide are established simultaneously, and 2) conversion of the fully methylated polysaccharide to derivatives suitable for separation and analysis is accomplished in a one-past work, we feel that the Reductive Cleavage Method has the potential to serve as a general method for the structural characterization of all complex carbohydrates, to include establishing the identities of constituent monomers, their ring form, their position(s) of linkage, their anomeric configuration, and their sequence. Experiments designed to examine the feasibility of this approach form the basis of this proposal. The research proposed herein therefore has as its objectives the integration of the Reductive Cleavage Method into a simplified scheme for complex carbohydrate structural analysis.