STRUCTURE AND FUNCTION OF MYELOPEROXIDASE

这个应用程序的目的是描绘分子 酶的作用机制。髓过氧化物酶，这是一种 中性粒细胞抗菌体系的主要成分 中性粒细胞。髓过氧化物酶的一个重要性质是它的 催化氢气生成次氯酸的能力 过氧化氢和氯离子杀死被摄取的有机体。首要目标 这项研究提案的主要内容是：(一)确定 髓过氧化物酶亚铁血红素基团 酶蛋白的蛋白水解性消化，(Ii) 血红素基团的特异性修饰对其结构和性能的影响 新型光化学修饰对髓过氧化物酶功能的影响 髓过氧化物酶的制备，以及(Iii)活性物质的详细阐明 髓过氧化物酶的位点结构及其与其他哺乳动物的比较 了解过氧化物酶的不同功能。我们 计划使用光吸收、共振拉曼、电子顺磁 磁共振、核磁共振和x射线光谱分析。自.以来 髓过氧化物酶是氧依赖抗微生物的中心酶 中性粒细胞系统，阐明该酶的分子机制 髓过氧化物酶的作用对于我们理解 中性粒细胞的杀菌功能，这是至关重要的 哺乳动物物种抵抗细菌感染的生存能力。

The objective of this application is to delineate the molecular mechanism of the enzyme action. of myeloperoxidase, which is one of the major components of the antimicrobial system of polymorphonuclear neutrophils. One of the important properties of myeloperoxidase is its ability to catalyze the formation of hypochlorous acid from hydrogen peroxide and chloride ion to kill the ingested organism. The primary goals of this research proposal are: (i) determination of the structure of the myeloperoxidase heme group by use of the heme-peptide prepared by proteolytic digestion of the enzyme protein, (ii) characterization of the effect of the specific modification of the heme group on the structure and function of myeloperoxidase by use of the novel photochemically modified myeloperoxidase preparation, and (iii) detailed elucidation of the active site structure of myeloperoxidase with reference to other mammalian peroxidase enzymes to understand the distinct function of this enzyme. We plan to use optical absorption, resonance Raman, electron paramagnetic resonance, nuclear magnetic resonance, and x-ray spectroscopy. Since myeloperoxidase is an central enzyme of oxygen dependent anti-microbial system of neutrophils, elucidation of the molecular mechanism of the enzyme action of myeloperoxidase is essential for our understanding the bactericidal function of polymorphonuclear leukocyte, which is vital for the survival of mammalian species against bacterial infections.