ANALYZE STEPS CONTROLLING GENE EXPRESSION IN DROSOPHILA

我们正在调查转录、加工和周转的贡献 对果蝇培养细胞和胚胎中特定基因的表达。 我们已经详细评估了这些参数对从 Copia序列，果蝇基因组中的一个可移动的DNA序列。为了 了解CopIA序列和移动DNA元件在总体上所起的作用 在细胞中发挥作用，我们现在正在研究Copia RNA的翻译产物。 为了比较COPIA序列的表达控制，这是 在培养的细胞中连续表达，并控制其表达 被诱导的基因，我们目前正在测量合成参数， 被诱导到高水平的两个基因的加工和周转 在热休克刺激下的表达。最后，我们已经证明了 组蛋白基因在果蝇胚胎中的表达水平受 通过改变合成速度和改变周转速度。我们目前正在 探讨组蛋白mRNA翻转的不同调控机制 可能会被控制住。

We are investigating the contributions of transcription, processing and turnover to the expression of specific genes in Drosophila cultured cells and embryos. We have evaluated these parameters in detail for the RNA transcribed from the copia sequence, a mobile DNA sequence in the Drosophila genome. In order to understand the role which the copia sequences and mobile DNA elements in general play in the cell, we are now investigating the translation product of copia RNA. In order to compare the control of expression of the copia sequence, which is expressed continuously in the cultured cells, with the control of expression of genes which are induced, we are currently measuring the parameters of synthesis, processing and turnover for two genes which are induced to a high level of expression by the stimulus of heat shock. Finally, we have demonstrated that the level of histone gene expression in Drosophila embryos is controlled both by changing rates of synthesis and by changing rates of turnover. We are currently investigating different mechanisms by which the control of histone mRNA turnover might be controlled.