MULTINUCLEAR NMR STUDIES OF CATION-DNA INTERACTIONS

这个项目的主要目标是 DNA上的多价阳离子结合环境及其作用机制 这些环境在生物进化的过程中会发生变化 重要的DNA结构转变，B-Z转变。 具体地说，提出了一系列59 Co核磁共振测量 命令(L)澄清钴的~(59)Co核磁共振弛豫行为 (NH3)63+与DNA结合，特别是对DNA的影响 DNA(2)表面阳离子迁移率检测碱基 组成、序列和DNA长度对Co(NH3)63+结合的影响 强度和异质性；(3)监测 Co(NH3)63+对合成DNA的B-Z转变的影响其他内容 建议使用13C和15N核磁共振测量来进行实验 阶(1)刻画了稀溶液的行为 特殊标记的多胺(2)以确定局部的pKA 多胺与DNA(3)的结合以表征多胺结合 到天然的、合成的和寡聚的DNA(4)来检查 捕获在DNA表面附近的多胺的迁移率和(5) 探索多胺参与B-Z转变。

The primary objectives of this project are to characterize multivalent cation binding environments on DNA and to explore how these environments change over the course of a biologically important DNA structural transition, the B-Z transition. Specifically, a series of 59 Co NMR measurements are proposed in order (l) to clarify the 59 Co NMR relaxation behavior of Co (NH3)63+ bound to DNA, in particular with respect to the influence of cation mobility on the surface of the DNA (2) to examine base composition, sequence and DNA length effects on Co(NH3)63+ binding strength and heterogeneity and (3) to monitor the state of Co(NH3)63+ over the B-Z transition of synthetic DNA. Additional experiments are proposed using 13C and 15N NMR measurements in order (1) to characterize the dilute-solution behavior of specifically labeled polyamines (2) to determine the local pKa's of polyamines bound to DNA (3) to characterize polyamine binding to natural, synthetic and oligomeric DNA's (4) to examine the mobility of polyamines trapped near the DNA surface and (5) to probe polyamine involvement in the B-Z transition.