GENETIC ANALYSIS OF CELL CYCLE-DEPENDENT TRANSCRIPTION

细胞增殖受到复杂的控制，其中之一 其中之一是细胞内基因的差异表达。 周而复始。在萌芽酵母中，酿酒酵母中， 一些编码酶的基因的周期性转录 DNA合成所需的是细胞进步的先决条件 通过S时相；组蛋白基因转录的限制 G1期晚期-S早期-也可能进化为调节酵母 细胞周期。控制差动的调节电路 将检测酵母组蛋白基因的转录情况。 影响细胞周期依赖转录的调节性突变 将通过筛选与表型相关的表型来识别 组蛋白-LacZ融合基因表达的改变。顺应时代 突变将被用来评估积极和消极的作用 周期转录中的组蛋白启动子元件。反演戏 突变导致构成转录过程中的 细胞周期将识别调控基因，这些基因需要 保持定期转录。调控基因将是 酵母突变体及其克隆基因的互补克隆 将被用于研究它们所在的蛋白质的功能 编码。

Cellular proliferation is subject to complex controls, one of which is the differential expression of genes during the cell cycle. In the budding yeast, Saccharomyces cerevisiae, the periodic transcription of a number of genes which encode enzymes required for DNA synthesis is a prerequisite for cells to progress through S-phase; the restriction of histone gene transcription to late G1-early S-phase may also have evolved to regulate the yeast cell cycle. The regulatory circuit which controls the differential transcription of the yeast histone genes will be examined. Regulatory mutations affecting cell cycle-dependent transcription will be identified by screening for phenotypes associated with the altered expression of histone-lacZ fusion genes. Cis-acting mutations will be used to assess the roles of positive and negative histone promoter elements in periodic transcription. Trans-acting mutations which result in constitutive transcription during the cell cycle will identify regulatory genes which are required to maintain periodic transcription. The regulatory genes will be cloned by complementation of yeast mutants and the cloned genes will be used to study the functions of the proteins which they encode.