CO-REGULATION OF N. CRASSA RIBOSOMAL RNA & PROTEIN GENES

该项目的总体目标是通过以下方式确定机制： 核糖体RNA和蛋白质基因的转录是协同的， 真核生物中的协调，使用粗糙脉孢菌作为模型 系统 此前，该实验室已经表明，粗糙脉孢菌5S和 40 SrRNA基因需要一个共同的序列，核糖框， 体外转录，链孢菌核糖体蛋白 crp-2基因在其5'端侧翼含有三个ribo盒 序列的 本提案旨在检验以下假设： 与核糖框结合的蛋白质 转录5S rRNA，40 S rRNA和核糖体蛋白基因， 脉孢菌 建议通过以下方式测试ribo框的调节作用： 将核糖体盒突变的rRNA和r蛋白基因 并测量它们的转录和调控 在体内，并通过将核糖框引入不相关的基因， 作为qa-2和tRNAleu，并测试这些添加物的效果 对这些基因在体内的调节。 审查 核糖体盒的分子作用，提出纯化核糖体盒。 核糖框结合蛋白并表征其性质，和 尝试克隆编码这种蛋白质的基因。

The overall aim of this project is to identify mechanisms by which the transcription of ribosomal RNA and protein genes is co- ordinated in eukaryotes, using Neurospora crassa as a model system. Previously this lab has shown that Neurospora crassa 5S and 40SrRNA genes require a common sequence, the ribo box, for transcription in vitro, and that a Neurospora ribosomal protein gene crp-2 contains three ribo boxes in its 5' flanking sequences. This proposal is designed to test the hypothesis that the protein that binds to the ribo box co-ordinates the transcription of 5S rRNA, 40S rRNA and ribosomal protein genes in Neurospora. It is proposed to test the regulatory role of the ribo box by placing rRNA and r-protein genes with ribo box mutations back into Neurospora and measuring their transcription and regulation in vivo, and by introducing ribo boxes into unrelated genes such as qa-2 and tRNAleu, and testing the effects of those additions on the regulation of those genes in vivo. To examine the molecular action of the ribo box it is proposed to purify the ribo box-binding protein and to characterize its properties, and to attempt the cloning of the gene coding for this protein.