REGULATION OF HIV MRNA 3' PROCESSING

描述：(改编自申请者的摘要)这项计划的中心目标 本项目旨在阐明其调控的生物化学基础。 HIV信使核糖核酸3‘处理。我们的长期目标是开发独特的 HIV聚(A)的性质现场信号处理设计有效 抗病毒剂。调查员建议找出原因 负责识别HIV Poly(A)位点上游分子和 将描述其增强处理的机制。 该上游因子的克隆及其功能鉴定 功能结构域将允许RNA结合的改变 该因素的专一性，以便于识别和后续 HIV 5‘核心聚(A)位点的加工。一种在体外很好地表征了 将利用处理系统来探索生物化学基础 HIV m RNA 3‘末端形成的调控。调查员有五个 具体的目标，其中第一个是试图确定过程 与HIV聚(A)位点上游元件相互作用的因子。下一首, 作者建议阐明上游因素的作用机制。 加强艾滋病毒多聚(A)部位处理的功能。第三，他会 尝试生化纯化HIV聚(A)位点上游因子并 分离相应的cDNA克隆。他还提议找出 介导序列识别和序列识别的上游因子结构域 功能以增强处理能力。最后，作者将尝试开发一个 嵌合加工因子和潜在的抗病毒药物，以促进 在HIV 5‘核心聚(A)位点进行处理。

DESCRIPTION: (Adapted from applicant's abstract) The central goal of this project is to try to elucidate the biochemical basis of the regulation of HIV mRNA 3' processing. The long-term objective is to exploit the unique nature of the HIV poly(A) site processing signal to design an effective antiviral agent. The investigator proposes to identify the factor responsible for recognition of the HIV poly(A) site upstream element and the mechanism by which it enhances processing will be delineated.The subsequent cloning of this upstream factor and the characterization of its functional domains will permit the alteration of the RNA-binding specificity of the factor in order to facilitate recognition and subsequent processing of the HIV 5' core poly(A) site. A well-characterized in vitro processing system will be employed to probe the biochemical basis of the regulation of HIV mRNA 3' end formation. The investigator has five specific aims the first of which is to try to identify the processing factor that interacts with the HIV poly(A) site upstream element. Next, the author proposes to elucidate the mechanism by which the upstream factor functions to enhance processing at the HIV poly(A) site. Third, he will attempt to purify biochemically the HIV poly(A) site upstream factor and to isolate the corresponding cDNA clones. He also proposes to identify the domains of the upstream factor that mediate sequence recognition and function to enhance processing. Lastly, the author will try to develop a chimeric processing factor, and potential antiviral agent, to facilitate processing at the HIV 5' core poly(A) site.