NEUROCHEMISTRY OF GESTATION-PRODUCED ANALGESIA

这个实验室以前的研究已经证明，在 怀孕时会出现一种激活的母体脊髓阿片类药物 特定阿片系统的中介系统(S)(S) 正在被调节的(阿片受体类型和内源性 激活它的配体)。具体的 目的：(1)确定脊髓阿片类药物的种类(S) 介导脊髓阿片类药物的受体(µ、β、kappa) 妊娠止痛。(2)测定内源性阿片类药物 配体(S)(脑啡肽、强啡肽、B-内啡肽)，介导 妊娠的脊髓止痛。(3)确定浓度 脑啡肽、强啡肽、β-内啡肽和总阿片类物质的研究概况 妊娠(第20天)和非妊娠大鼠脊髓超融合液中的活性 怀孕的老鼠。除上述外，实验还将 进行以确定怀孕的各方面(S)(在 外周荷尔蒙浓度，压力增加，对抗 子宫颈等。)这是增长的原因 妊娠期间脊髓阿片类药物活动。具体目标 将确定(4)止痛是否与 假孕涉及一种脊髓阿片成分，就像 与实际怀孕相关的止痛，以及(5) 确定施加于子宫的力量增加的效果 宫颈对非妊娠女性和假孕妇女痛觉阈值的影响 老鼠。这项研究将表明，如果对 子宫颈在怀孕期间自然发育的子宫颈 分娩可能至少是导致分娩的内部刺激之一 在母亲阿片系统的激活中(S)及其伴随 痛觉阈值升高。

Previous studies in this laboratory have demonstrated that during pregnancy there occurs an activation of a maternal spinal opioid system(s) that mediated characterize the specific opioid system(s) that is being modulated (type of opioid receptor and endogenous ligand that activates it) during gestation. The specific objectives are to (1) Determine the type(s) of spinal opiate receptor (mu, delta, kappa) that mediates the spinal opioid analgesia of pregnancy. (2) Determine the endogenous opioid ligand(s) (enkephalin, dynorphin, B-endorphin) that mediates the spinal analgesia of pregnancy. (3) Determine the concentration profile of enkephalin, dynorphin, B-endorphin and total opioid activity in spinal cord superfusate of pregnant (day 20) and non- pregnant rats. In addition to the above, experiments will be conducted to determine the facet(s) of pregnancy (changes in peripheral hormone concentration, increased pressure against the uterine cervics, etc.) that is responsible for the increase in spinal opioid activity during gestation. The specific objectives will be to (4) Determine if the analgesia associated with pseudopregnancy involves a spinal opioid component as does the analgesia that is associated with actual pregnancy, and (5) Determine the effect of increased force applied to the uterine cervix on pain thresholds on non-pregnant female and pseudopregnant rats. This study will indicate if the increase pressure against the uterine cervix that develops naturally during pregnancy and labor might be at least one of the internal stimuli that results in the activation of maternal opioid system(s) and the concomitant elevation in pain threshold.