NEUROCHEMISTRY OF GESTATION-PRODUCED ANALGESIA

拟议研究的总体目标是确定 外周和中央突起负责抬高 对观察到的厌恶刺激的反应阈值 在大鼠怀孕期间。将使用的一种方法是 确定模仿和操作 妊娠状态对大鼠痛阈值的影响。第二种方法将 测定母血和脑脊液(CSF) 妊娠期间阿片肽的浓度分布。具体的 目标是(1)确定雌激素、黄体酮是否有作用。 和催乳素，单独和联合使用，对疼痛阈值 非孕去卵巢大鼠。(2)确定高程是否位于 在假孕期间观察痛觉阈值，包括有无疼痛 蜕膜瘤。(3)确定宫内压力增加和 扩张足以引起痛觉阈值的增加 激素对假孕大鼠和去卵巢大鼠的影响 治疗。(4)确定是否存在发育中的胎儿 对于提高产妇的疼痛阈值来说是必不可少的。(5)确定 母血中β-内啡肽和总阿片的血药浓度分布 妊娠期和产后的多肽。以上内容 实验将使评估外围进程成为可能 参与妊娠引起的痛觉阈值升高。(6)在 为了评估中枢机制在妊娠诱导中的作用 产妇脑脊液中β-内啡肽的浓度分布， 甲硫氨酸和亮氨酸脑啡肽和总阿片类药物将被测定 在怀孕期间。(7)上述操纵的影响及 模拟妊娠对母体血液和脑脊液阿片类药物水平的影响 还将对多肽进行调查。

The overall objective of the proposed research is to determine the peripheral and central processes that are responsible for the elevation in the threshold for responsiveness to aversive stimuli that is observed during gestation in rats. One approach that will be utilized will be to determine the effects of mimicking and manipulating specific aspects of the pregnant condition on the pain threshold in rats. A second approach will be to determine the materal blood and cerebrospinal fluid (CSF) concentration profile of opioid peptides during gestation. The specific objectives are to (1) Determine the role, if any, of estrogen, progesterone and prolactin, alone and in combination, on the pain threshold of nonpregnant ovariectomized rats. (2) Determine whether an elevation in pain threshold is observed during pseudopregnancy, with and without deciduoma. (3) Determine if an increase in intrauterine pressure and distention is sufficient to produce an increase in pain threshold in pseudopregnant rats and ovariectomized rats subjected to hormone treatment. (4) Determine whether the presence of a developing fetus(es) is essential for the elevation in maternal pain threshold. (5) Determine the maternal blood concentration profile of B-endorphin and total opioid peptides during gestation and the post partem period. The above experiments will enable the evaluation of peripheral processes that might be involved in the pregnancy-induced elevation in pain threshold. (6) In order to assess the involvement of central mechanisms in pregnancy-induced analgesia the maternal CSF concentration profile of B-endorphin, methionine- and leucine-enkephalin and total opioids will be determined during gestation. (7) The effects of the above manipulations and simulations of pregnancy on maternal blood and CSF levels of opioid peptides will also be investigated.