MODE OF ACTION OF 6-MBOA AS A REPRODUCTIVE STIMULATOR

6-甲氧基苯并恶唑烷酮(6-MBOA)是一种最初从 自然生长的植物，刺激所有哺乳动物的生殖，所以 久经考验。6-MBOA引起两性性腺激素肥大 微齿啮齿动物蒙太斯田鼠(脊椎动物)与 加大生育力度。6-姆博阿政府克服了 光周期对蒙古根结线虫的抑制作用，并且似乎 在提示生殖行为方面比光周期更重要。 6-MBOA现在被公认为是一种生殖刺激剂和生育 增强剂；然而，关于它的作用机制，在 内分泌或生化水平。我们的初步研究表明，6-mboa 是一种褪黑素拮抗剂，因为它竞争性地取代了~3H-褪黑素 它与蛋白质受体特异性结合。褪黑素的影响 在人类和其他动物中的性发育，很可能是生物学上的 影响心理抑郁的因素。 本提案中描述的研究旨在作为 6-MBOA对神经内分泌影响的综合研究 在细胞和生化水平上的系统及其作用机制(S)。 这里描述的项目的目标是：(1)确定主要的 通过测量组织分布来获得6-MBOA的次级靶组织 和体外结合，(2)测定促性腺激素黄体生成素和卵泡刺激素的变化 6-MBOA给药后用放射免疫法测定(3) 6-Mboa对某些血清类固醇水平的影响；(4)探讨可能的6-Mboa 以及褪黑素在已建立的靶组织中介导的生化变化。 将使用的方法是既定的和客观的。结果是 确定6-MBOA对神经内分泌系统的影响。

6-Methoxybenzoxazolinone (6-MBOA) is a compound originally isolated from naturally growing plants which stimulates reproduction in all mammals so far tested. 6-MBOA causes gonadol hypertrophy in both sexes of the microtine rodent Microtus montanus (vertebrates) with a commensurate increase in reproductive efforts. 6-MBOA administration overcomes the suppressive effects that photoperiods have on M. montanus, and appears to be more important than photoperiods in cueing reproductive behavior. 6-MBOA is now well established as a reproductive stimulant and fertility enhancer; however, there is no information on its mechanism of action at the endocrine or biochemical level. Our initial studies show that 6-MBOA is a melatonin antagonist in that it competitively displaces 3H-melatonin which is specifically bound to protein receptors. Melatonin influences sexual development in humans and other animals and is likely a biological factor influencing psychological depression. Research described in this proposal is intended as the initial phase of a comprehensive study to define effects of 6-MBOA on the neuroendocrine system and its mechanism(s) of action at the cellular and biochemical level. The objectives of the project described here are to: (1) determine primary and secondary target tissues for 6-MBOA by measuring tissue distributions and in vitro binding, (2) measure changes in the gonadotropins LH and FSH by radioimmunoassay after 6-MBOA administration, (3) determine effects of 6-MBOA on the levels of some serum steroids, and (4) study possible 6-MBOA and melatonin-mediated biochemical changes in established target tissues. Methodologies to be used are well established and objective. Results are certain to determine effects of 6-MBOA on the neuroendocrine system.