NEUROCHEMISTRY OF GESTATION-PRODUCED ANALGESIA

拟议研究的总体目标是确定 周边和中央过程，负责海拔在 观察到的对厌恶刺激的反应阈值 在大鼠妊娠期间。 将采用的一种方法是 确定模仿和操纵的特定方面的影响， 妊娠状态对大鼠痛阈的影响。 第二种方法将 是为了确定母血和脑脊液（CSF） 妊娠期间阿片肽的浓度曲线。 具体 目的是（1）确定雌激素、孕激素的作用，如果有的话， 和催乳素，单独和联合使用，对 非妊娠卵巢切除大鼠。 (2)确定是否在 疼痛阈值观察期间的假睡眠，有和没有 蜕膜瘤 (3)确定子宫内压是否升高， 膨胀足以使疼痛阈值增加， 假孕大鼠和卵巢切除大鼠接受激素 治疗 (4)确定是否存在发育中的胎儿， 这对提高产妇的疼痛阈值至关重要。 (5)确定 B-内啡肽和总阿片类药物的母体血液浓度曲线 肽在妊娠期间和产后期间。 上述 实验将能够评估可能 与妊娠引起的痛阈升高有关。 (6)在 为了评估中枢机制在妊娠诱导的 镇痛母体CSF中β-内啡肽的浓度分布， 将测定蛋氨酸-和亮氨酸-脑啡肽和总阿片类药物 在怀孕期间 (7)上述操作的效果和 模拟妊娠对母体血液和CSF阿片类药物水平的影响 还将研究肽。

The overall objective of the proposed research is to determine the peripheral and central processes that are responsible for the elevation in the threshold for responsiveness to aversive stimuli that is observed during gestation in rats. One approach that will be utilized will be to determine the effects of mimicking and manipulating specific aspects of the pregnant condition on the pain threshold in rats. A second approach will be to determine the materal blood and cerebrospinal fluid (CSF) concentration profile of opioid peptides during gestation. The specific objectives are to (1) Determine the role, if any, of estrogen, progesterone and prolactin, alone and in combination, on the pain threshold of nonpregnant ovariectomized rats. (2) Determine whether an elevation in pain threshold is observed during pseudopregnancy, with and without deciduoma. (3) Determine if an increase in intrauterine pressure and distention is sufficient to produce an increase in pain threshold in pseudopregnant rats and ovariectomized rats subjected to hormone treatment. (4) Determine whether the presence of a developing fetus(es) is essential for the elevation in maternal pain threshold. (5) Determine the maternal blood concentration profile of B-endorphin and total opioid peptides during gestation and the post partem period. The above experiments will enable the evaluation of peripheral processes that might be involved in the pregnancy-induced elevation in pain threshold. (6) In order to assess the involvement of central mechanisms in pregnancy-induced analgesia the maternal CSF concentration profile of B-endorphin, methionine- and leucine-enkephalin and total opioids will be determined during gestation. (7) The effects of the above manipulations and simulations of pregnancy on maternal blood and CSF levels of opioid peptides will also be investigated.