HUMAN THROMBINS

凝血酶已成为研究最好的丝氨酸蛋白酶之一， 酶（蛋白水解）和非酶（激素）生物调节 在止血、伤口愈合和相关生理过程中的功能。 大约有1000万到2000万单位的人类α-凝血酶， 和所有其他凝血酶归因的活动继续产生在我们的 我们每年都在实验室进行自己的研究，也为其他国家的研究提供服务。 实验室（美国和国外）。 除了这种形式，我们还 缺乏凝血活性的酶活性形式（例如，通过自溶， 胰蛋白酶、弹性蛋白酶消化）和酶失活形式 激素活性（例如，通过用i-Pr 2 P-F修饰， D-Phe-Pro-Arg-Ch 2-Cl），以绘制活性位点区域和结构 酶和非酶功能所需的凝血酶结构域。 我们 还试图分离出具有生物活性的片段（例如，白细胞 蛋白酶抑制剂），其可能参与凝血后事件。 合作研究为我们提供了评估各种 生物活性（例如，血小板反应，血管内皮 单层通透性、白细胞功能）。 其他合作研究 还将包括人（原）凝血酶重组基因的评价 产品. 我们的扩展实验室方法被认为已经导致了 主要贡献（例如，大部分材料最近 ISTH第十届大会关于“蜂窝事件”的卫星专题讨论会 由凝血酶和相关蛋白酶介导”（圣地亚哥，1985年7月）和 纽约科学院会议“生物调节功能 凝血酶”（纽约，1986年2月5-7日）。

Thrombin(s) has become among the best studied serine proteinases and has both enzymic (proteolytic) and nonenzymic (hormonal) bioregulatory functions in hemostasis, wound healing, and related physiologic processes. Some 10- to 20- million units of human Alpha-thrombin with high clotting and all other thrombin-ascribed activities continue to be produced in our laboratory annually for our own studies, as well as for those in other laboratories (U.S. and abroad). In addition to this form, we are making enzymically active forms lacking clotting activity (e.g., by autolytic, tryptic, elastolytic digestion) and enzymically inactivated forms posessing hormonal activities (e.g., by modification with i-Pr2P-F, D-Phe-Pro-Arg-Ch2-Cl) in order to map active-site regions and structural domains of thrombin required for enzymic and nonenzymic functions. We are also attempting to isolate hormonally active fragments (e.g., leucocyte proteinase digests), which might participate in post clotting events. Collaborative studies provide us with the ability to assess various biological activities (e.g., platelet responses, vascular endothelial monolayer permeability, leucocyte functions). Other collaborative studies will also include the evaluation of human (pro)thrombin recombinant gene products. Our extended laboratory approach is believed to have resulted in major contributions (e.g., the majority of materials for the recent Satellite Symposium of the Xth Congress of the ISTH on "Cellular Events Mediated by Thrombin and Related Proteinases" (San Diego, July 1985) and the New York Academy of Sciences Conference on "Bioregulatory Functions of Thrombin" (New York, February 5-7, 1986).