ROLE OF PLATELET LIPIDS IN HEMOSTASIS AND THROMBOSIS

本提案探讨了花生四烯酸含醚的作用 磷脂在血小板功能中的作用 该提案的主要重点是 研究（1）血小板转酰酶，负责 用于将花生四烯酸掺入醚磷脂中，和（2） 血小板磷脂酶A2，从 用于随后转化为生物活性的磷脂 代谢物。 该提案的主要目标是探索控制和 花生四烯酸在乙醚中代谢的功能意义 人体血小板中的磷脂 主要技术将包括方法 用于纯化和分析膜相关酶（去污剂 溶解、柱色谱法、电泳）以及用于 脂质混合物的分辨率和分析（薄层和高性能 液相色谱法）。 这些研究将（1）提供深入了解生物化学和功能 参与生物活性物质生产的酶的性质 化合物（类花生酸和血小板活化因子），（2）阐明 醚磷脂的生物学作用和（3）进一步了解 血小板功能和血小板参与出血和 血栓性疾病

This proposal examines the role of arachidonic acid containing ether phospholipids in platelet function. The major focus of the proposal is to study regulation and properties of (1) platelet transacylase, responsible for incorporation of arachidonic acid into ether phospholipids and (2) platelet phospholipase A2, liberating esterified arachidonate from phospholipids for subsequent transformation to biologically active metabolites. The major goal of the proposal is to explore the control and functional significance of the metabolism of arachidonic acid in ether phospholipids in human platelets. Major techniques will include methods for purification and analysis of membrane-associated enzymes (detergent solubilization, column chromatography, electrophoresis) and methods for resolution and analysis of lipid mixtures (thin layer and high performance liquid chromatography). These studies will (1) provide insight into biochemical and functional properties of enzymes involved in production of biologically active compounds (eicosanoids and platelet activating factor), (2) elucidate the biological role(s) of ether phospholipids and (3) further the knowledge of platelet function and involvement of platelets in hemorrhagic and thrombotic diseases.