GENETIC CONTROL OF EARLY MAMMALIAN DEVELOPMENT

仍然只有有限的关于基因的信息 哺乳动物早期胚胎发育的控制。长期的 此应用程序的目标是利用新技术 外源DNA种质导入鉴定及突变研究 参与控制早期细胞谱系决定的基因 小鼠胚胎。这样一种突变和分子遗传的结合 分析为遗传分析提供了一种强有力的新方法 发展，将对以下方面产生重大影响 了解人类正常胚胎发育及其过程 这会导致胎儿损耗和发育异常。这个 这项建议的具体目的是推行三项战略 在识别和突变参与早期发育的基因方面。 目的1：继续筛选新的转基因小鼠品系 发育突变，以表征突变和克隆 野生型基因。 目标2：识别并可能突变ES中活跃的新基因 LacZ基因报道的细胞或原肠胚期胚胎 技巧。 目标3：开发高效的同源基因检测技术 在ES细胞中重组并由此突变N-myc和Hox 2.1 意思是。

There is still only limited information available on the genetic control of early embryonic development in mammals. The long-term objective of this application is to utilize new technologies of germ-line introduction of exogenous DNA to identify and mutate genes involved in controlling early cell lineage decisions in the mouse embryo. Such a combined mutational and molecular genetic analysis provides a powerful new approach to genetic analysis of development and will have considerable implications for understanding normal human embryonic development and the processes that lead to fetal wastage and developmental abnormalities. The specific aim of this proposal is to pursue three strategies aimed at identifying and mutating genes involved in early development. Aim 1: To continue to screen transgenic mouse strains for new developmental mutations, to characterize the mutations and clone the wild-type genes. Aim 2: To identify and possibly mutate new genes active in ES cells or gastrulation-stage embryos by lacZ "gene-reporter" techniques. Aim 3: To develop efficient techniques for homologous recombination in ES cells and mutate N-myc and Hox 2.1 by this means.