REGULATION OF SMOOTH MUSCLE CONTRACTION

这个研究项目的目标是阐明一些基本的 控制冠状动脉收缩和增重的机制 对药剂可以松弛平滑肌的机制的洞察。 具体地说，该应用程序建议确定是否有一个角色 蛋白激酶C在控制平滑肌收缩中的作用。这个 研究人员将确定特定的磷酸化模式 蛋白激酶C的活性，并使用这些模式来监测 该激酶参与控制Smooth收缩的事件 肌肉。此外，他们将确定刺激剂是否对 活性佛波醇酯的平滑肌是由于这些药物的作用。 他们还将开发一种“最先进”的检测方法 二酰基甘油，灵敏和准确，可用于小型 一条条光滑的肌肉。这项测试将被用来调查 二甘油浓度变化控制蛋白质的可能性 肌动蛋白C的活性，并确定其中的一些因素 可以改变完好的平滑肌中的甘油二酯含量。

The goals of this research program are to elucidate some of the fundamental mechanisms that control contraction of coronary arteries and to gain insights into the mechanisms by which agents can relax smooth muscle. Specifically, this application proposes to determine if there is a role for protein kinase C in the control of smooth muscle contraction. The investigators will identify specific phosphorylation patterns that are due to protein kinase C activity and use these patterns to monitor the involvement of this kinase in the events that control contraction of smooth muscle. In addition, they will determine if the stimulant effects on smooth muscle of active phorbol esters are due to effects of these agents on protein kinase C. They will also develop a "state of the art" assay for diacylglycerol that is sensitive and accurate enough for use in small strips of smooth muscle. This assay will be utilized to investigate the possibility that changes in diaclyglycerol concentration control protein kinase C activity in smooth muscle, and to determine some of the factors that can alter diaclyglycerol content of intact smooth muscle.