MAPPING & SEQUENCING THE HUMAN Y CHROMOSCOME

这个项目的重点是人类Y染色体的背景下， 基因组计划。该项目的主要长期目标已经完成 人类Y染色体的遗传内容的定义。朝向 最后，构建染色体的详细遗传缺失图谱， 跨越染色体的重叠重组DNA克隆将被 分离，并确定染色体的核苷酸序列。 在这项工作的初期，将高度优先重视 使用自动化技术开发高效的DNA测序策略 仪器.由此产生的信息将提供一个框架， 阐明了Y染色体在各种过程中的功能， 精子发生、肿瘤发生、胚胎体细胞和性细胞 发展这些研究也将揭示许多关于 人类X染色体之间的功能、结构和进化关系 和Y染色体。

This project focuses on the human Y chromosome in the context of the Human Genome Initiative. The principal long-term goal of this project is complete definition of the genetic content of the human Y chromosome. Toward that end, a detailed genetic deletion map of the chromosome will be constructed, overlapping recombinant DNA clones spanning the chromosome will be isolated, and the nucleotide sequence of the chromosome will be determined. During the early course of this work, high priority will be given to the development of efficient DNA sequencing strategies using automated instruments. The resulting information will provide a framework for elucidating the function of the Y chromosomes in processes as diverse as spermatogenesis, tumorigenesis, and embryonic somatic and sexual development. These studies will also reveal much about the complex functional, structural, and evolutionary relationship between the human X and Y chromosomes.