GENETIC CONTROL OF EARLY MAMMALIAN DEVELOPMENT

目前关于遗传学的信息仍然有限。 哺乳动物早期胚胎发育的控制。 长期 本申请的目的是利用新技术， 外源DNA的种系导入以鉴定和突变 参与控制早期细胞谱系决定的基因 小鼠胚胎 这种突变和分子遗传的结合 分析提供了一个强大的新方法，遗传分析， 发展，并将产生重大影响， 了解正常的人类胚胎发育和过程 会导致胎儿的浪费和发育异常 的 这项建议的具体目标是实施三项战略， 识别和突变与早期发育有关的基因。 目的1：继续筛选新的转基因小鼠品系 发育突变，以表征突变和克隆 野生型基因。 目的2：鉴定并可能突变ES中的新基因 细胞或原肠期胚胎的lacZ“基因报告” 技术. 目的3：开发高效的同源重组技术， 在ES细胞中重组，并通过这种重组突变N-myc和Hox 2.1。 手段

There is still only limited information available on the genetic control of early embryonic development in mammals. The long-term objective of this application is to utilize new technologies of germ-line introduction of exogenous DNA to identify and mutate genes involved in controlling early cell lineage decisions in the mouse embryo. Such a combined mutational and molecular genetic analysis provides a powerful new approach to genetic analysis of development and will have considerable implications for understanding normal human embryonic development and the processes that lead to fetal wastage and developmental abnormalities. The specific aim of this proposal is to pursue three strategies aimed at identifying and mutating genes involved in early development. Aim 1: To continue to screen transgenic mouse strains for new developmental mutations, to characterize the mutations and clone the wild-type genes. Aim 2: To identify and possibly mutate new genes active in ES cells or gastrulation-stage embryos by lacZ "gene-reporter" techniques. Aim 3: To develop efficient techniques for homologous recombination in ES cells and mutate N-myc and Hox 2.1 by this means.