REGULATION OF SMOOTH MUSCLE CONTRACTION

这项研究计划的目标是阐明一些基本的 控制冠状动脉收缩的机制， 深入了解药物放松平滑肌的机制。 具体地，该应用程序提出确定是否存在用于 蛋白激酶C控制平滑肌收缩。 的 研究人员将确定特定的磷酸化模式， 蛋白激酶C活性，并使用这些模式来监测 这种激酶参与控制平滑肌收缩的事件， 肌肉. 此外，他们还将确定兴奋剂是否对 活性佛波醇酯平滑肌是由于这些试剂的作用 对蛋白激酶C的影响 他们还将开发一种“最先进”的检测方法， 二酰基甘油，其灵敏度和准确度足以用于小型 平滑肌条 本试验将用于研究 二酰基甘油浓度控制蛋白的变化 平滑肌激酶C活性，并确定一些因素 可以改变完整平滑肌中甘油二酯的含量。

The goals of this research program are to elucidate some of the fundamental mechanisms that control contraction of coronary arteries and to gain insights into the mechanisms by which agents can relax smooth muscle. Specifically, this application proposes to determine if there is a role for protein kinase C in the control of smooth muscle contraction. The investigators will identify specific phosphorylation patterns that are due to protein kinase C activity and use these patterns to monitor the involvement of this kinase in the events that control contraction of smooth muscle. In addition, they will determine if the stimulant effects on smooth muscle of active phorbol esters are due to effects of these agents on protein kinase C. They will also develop a "state of the art" assay for diacylglycerol that is sensitive and accurate enough for use in small strips of smooth muscle. This assay will be utilized to investigate the possibility that changes in diaclyglycerol concentration control protein kinase C activity in smooth muscle, and to determine some of the factors that can alter diaclyglycerol content of intact smooth muscle.