CHOLESTERYL ESTER LEVELS IN MAMMALIAN TISSUES

这项研究的长期目标是了解 游离胆固醇和酯化胆固醇的分布受到调节， 在组织和细胞内池之间。 这个项目是 涉及整个过程的具体监管方面。 主要关注的是界面反应的调节。 胆固醇酯+水与脂肪的平衡 酸+胆固醇 由哺乳动物胆固醇酯酶催化。 与此相关 是调节脂质底物的自发转运 以及产物进出反应位点。 在这方面，委员会注意到， 游离胆固醇分配到水相中， 胆固醇和胆固醇酯穿过双层的运动将 被解决。 水解的一个重要特征是 反应和传输反应的区别在于它们是界面反应。 因此，主要的实验重点是脂质的作用- 脂质和脂质-蛋白质相互作用的表面相， 这些过程的规范。 具体而言，酶吸附到和 氩气缓冲液中表面相底物水解 接口将被测量。 使用的酶是 胰羧酸酯脂肪酶，溶酶体胆固醇酯酶 和激素敏感性胆固醇酯酶。 重点是 确定这些酶是如何被特定的脂质调节的， 脂质相互作用 从结果中获得明显的酶 特异性可分为物理和化学 特殊性 自发性神经元的调控研究 胆固醇和胆固醇酯跨双层的转移将 揭示在什么条件下胆固醇酯酶可以促进 胆固醇在双层中的移动。 这些目标的实现将大大提高我们的 了解胆固醇分布的调节 在体内的化学形式和物理池之间。

The long-term goal of this research is to understand how the distribution of free and esterified cholesterol is regulated, both among tissue and among intracellular pools. This project is concerned with specific regulatory aspects of the overall process. Primary focus is on the regulation of the interfacial reaction. CHOLESTERYL ESTER + WATER EQUILIBRIUM WITH FATTY ACID + CHOLESTEROL as catalyzed by mammalian cholesterol esterases. Related to this is the regulation of the spontaneous transport of lipid substrate and products to and away from the reaction site. In this regard, the partitioning of free cholesterol into the aqueous phase and the movement of cholesterol and cholesteryl ester across bilayers will be addressed. An important characteristic of both the hydrolysis reaction and the transport reactions is that they are interfacial. Hence, the major experimental emphasis is on the role of lipid- lipid and lipid-protein interactions in surface phases in the regulation of these processes. Specifically, rates and extents of enzyme adsorption to and substrate hydrolysis in surface phases at the argon-buffer interface will be measured. The enzymes to be used are pancreatic carboxylester lipase, lysosomal cholesterol esterase and hormone sensitive cholesterol esterase. Emphasis is on determining how these enzymes are regulated by specific lipid- lipid interactions. From the results obtained apparent enzyme specificity can be separated into physical and chemical specificities. Studies of the regulation of the spontaneous transfer of cholesterol and cholesteryl ester across bilayers will reveal under what conditions cholesterol esterases may facilitate cholesterol movement across bilayers. The achievement of these aims will greatly increase our understanding of the regulation of the distribution of cholesterol between chemical forms and physical pools in vivo.