TRACHEOBRONCHIAL GLYCOPROTEIN STRUCTURE

在这项建议中，我们的目的是继续我们的研究努力，以 纯化的人的化学和结构特征 气管、支气管糖蛋白。主要重点放在 主要硫化低聚糖的分析及主要 大豆低聚糖-寡肽亚基的分离 气管、支气管糖蛋白。这项提案的重点是 重点介绍了硫酸化低聚糖的表征 因为主要是这一部分随着疾病的严重程度而增加 肺部疾病。气管、支气管糖蛋白及其分离纯化 来自不同形式慢性精神分裂症患者的部分 阻塞性肺疾病(如慢性支气管炎、哮喘、 支气管扩张症、肺泡蛋白沉积症和囊性纤维化 被研究，阐明和比较它们的结构，以便获得 来自不同病理载体的洞察如何 气管-支气管肌下腺改变其分泌 糖蛋白结构对慢性病的反应。自.以来 在最初的建议开始时，我们已经做出了实质性的和 多种硫酸盐类化合物结构研究的重要进展 低聚糖。我们计划继续这项令人兴奋的工作，在 除了加强研究工作外，我们还分析了 大豆杉低聚糖-寡肽不同组分的分离 气管、支气管糖蛋白。气-液色谱和 所有的化学研究都将采用质谱仪。 它还将包括几种新的分析技术 在最初的建议书期间开发，用于分析 氨基酸和中性糖、氨基酸和羧酸盐。 我们对其结构和相关功能的了解 气管、支气管糖蛋白仍然很差。在过去的时间里 两年来，我们取得了重大发现；发现了几个 新的硫酸盐化结构。我们正逐渐开始瓦解 这些复杂的、大分子量的基本结构 糖蛋白。继续这项研究很重要，这样才能 我们可以了解这些化合物的结构/功能生化 在健康和疾病中非常重要的分子。

Our aim, in this proposal, is to continue our research efforts to chemically and structurally characterize purified human tracheobronchial glycoproteins. Primary emphasis is placed on analyzing the major sulfated oligosaccharides and the major oligosaccharide-oligopeptide subunits isolated from tracheobronchial glycoproteins. The focus of this proposal stresses the characterization of the sulfated oligosaccharides since it is primarily this fraction that increases with severity of a lung disease. Tracheobronchial glycoproteins and isolated fractions from patients suffering from various forms of chronic obstructive pulmonary disease (e.g., chronic bronchitis, asthma, bronchiectasis, alveolar proteinosis and cystic fibrosis (CF)) will be studied, their structures elucidated and compared so as to gain insight from different pathological vectors on how the tracheobronchial submusocal glands alter their secretory glycoprotein structure in response to chronic disease. Since the start of the initial proposal, we have made substantial and significant progress in elucidating the structures of many sulfated oligosaccharides. We propose to continue this exciting work, in addition to enhancing our research efforts in analyzing the different oligosaccharide-oligopeptide fractions isolated from tracheobronchial glycoproteins. Gas-liquid chromatography and mass spectrometry will be employed in all chemical investigations which will also include several new analytical techniques developed during the initial proposal period for the analysis of amino and neutral sugars, amino acids and carboxylates. Our knowledge of the structure and related functions of tracheobronchial glycoproteins is still very poor. Over the past two years we have made significant findings; discovering several new sulfated structures. We are gradually beginning to unravel the basic structure of these complex, large molecular weight glycoproteins. Continuation of this research is important so that we can understand the structure/function biochemistry of these very important molecules in health and disease.