DETECTION OF THE CARRIER STATE OF AIDS

有证据表明艾滋病可能通过输血传播， 无症状艾滋病高危人群的成员促使寻找一个 筛选测试，可用于识别携带者状态， disorder. 我们建议评估选定的替代检测方法， 预测艾滋病或持续性淋巴结病发展的能力 综合征在一组同性恋男子的风险增加，发展 这些紊乱。 我们还将研究两组志愿献血者 实验室检查结果在我们的普通供体库中并不常见，但 已知在艾滋病患者和艾滋病患者中 相关障碍。 将在3年内对人群进行系列研究， 包括150名表面上健康同性恋男子， 性伴侣，100名自愿献血者， 辅助性/诱导性T淋巴细胞与抑制性/细胞毒性T淋巴细胞的比例（Th：Ts）， 100名志愿献血者，带有人类T细胞抗体 白血病/淋巴瘤病毒（HTLV）和50名Th：Ts志愿献血员 在正常范围内。 5例完全表达的艾滋病患者组成的组 和50名持续性全身淋巴结病患者将在 只是研究的开始。 除了临床随访外， 将在研究开始时和 6个月间隔后：使用 一组单克隆抗体，包括几种新型试剂; 血清中可溶形式的血小板表面分子的测量; 检测三种已识别的人类T细胞抗体的测试 白血病淋巴瘤病毒（HTLV）;循环免疫复合物试验; β 2-微球蛋白和胸腺素α 1的血清水平; 存在针对B型肝炎核心抗原（HBcAb）的抗体。 这些测定 在令人鼓舞的初步研究的基础上， 和潜在的方法论的简易性。 临床与 艾滋病高危人群和献血人群的实验室检查结果可能会有所帮助 确定最能预测的程序或程序组合 艾滋病或相关疾病的后续发展，有可能 应用于筛查捐献的血液以检测艾滋病携带者状态。

Evidence that AIDS may be transmitted by transfusion of blood from asymptomatic members of AIDS high risk groups has prompted a search for a screening test that can be used to identify a carrier state for the disorder. We propose to assess selected alternative assays for their ability to predict the development of AIDS or persistent lymphadenopathy syndrome in a group of homosexual men at increased risk for developing these disorders. We will also study two groups of volunteer blood donors with laboratory findings seen uncommonly in our general donor pool, but known to be significantly more prevalent in patients with AIDS and AIDS related disorder. The populations to be studied serially over 3 years will include 150 apparently healthy homosexual men with multiple anonymous sexual partners, 100 volunteer blood donors with persistent inversions of the ratio of helper/inducer to suppressor/cytotoxic T lymphocytes (Th:Ts), 100 volunteer blood donors with antibodies to the human T cell leukemia/lymphoma viruses (HTLV), and 50 volunteer blood donors with Th:Ts within the normal range. Groups of 5 patients with fully expressed AIDS and 50 with persistent generalized lymphadenopathy will be tested at the beginning of the study only. In addition to clinical follow-up, the following assays will be performed at the initiation of the study and at six month intervals thereafter: analysis of T lymphocyte subsets using a panel of monoclonal antibodies, including several novel reagents; measurement in serum of soluble forms of lympohcyte surface molecules; tests to detect antibodies to the three identified types of human T cell leukemia lymphoma viruses (HTLV); tests of circulating immune complexes; serum levels of beta-2-microglobulin and thymosin alpha-1; and tests for the presence of antibody to hepatitis B core antigen (HBcAb). These assays have been chosen for study on the basis of encouraging preliminary studies and potential ease of methodology. Correlation of the clinical and laboratory findings in the AIDS hight risk and blood donor groups may help to identify the procedure or combination of procedures best able to predict subsequent development of AIDS or related disorders, with potential applications to screening donated blood to detect an AIDS carrier state.