PULMONARY EXTRACELLULAR MATRIX

大鼠肺泡基底膜的合成将在 三种不同的情况：（a）在新生儿肺泡成熟期间 隔膜，（B）当新鲜分离的肺细胞II在 培养，和（c）在修复短期吸入性损伤的过程中， 肺泡内层 在初步实验中，我们发现， 新生大鼠肺基底膜放射性氨基酸掺入 膜前胶原IV，我们提取和鉴定。 同样我们 应研究其他细胞外基质成分的形成， 它们之间以及与细胞之间的相互作用。 一个假说是 建议将细胞表面与相邻细胞表面的相互作用 细胞迁移过程中的细胞外基质，并在制定 基底膜 为了研究这一点，将分离肺细胞II 并在几种条件下培养。 预涂餐具的效果 与基底膜成分的关系将被测试， 将探索细胞分裂到细胞外基质合成。 的 基底膜合成和降解的潜在相关作用 在修复牙槽膜的过程中，将进行评估。 总研究 将提供深入了解肺泡之间的生物合成关系， 细胞和它们制造和粘附的基底膜。

The synthesis of rat alveolar basement membranes will be compared under three different conditions: (a) during neonatal maturation of alveolar septa, (b) when freshly isolated pneumocytes II make these components in culture, and (c) during repair of short term inhalation damage of the alveolar lining. In preliminary experiments we found that slices of neonatal rat lung incorporated radioactive amino acids into basement membrane procollagen IV, which we extracted and identified. Similarly, we shall study the formation of other extracellular matrix components and their interactions with each other and with cells. A hypothesis is proposed to relate interactions of cell surfaces with adjacent extracellular matrix during cell migration and in the elaboration of basement membranes. To investigate this, pneumocytes II will be isolated and cultured under several conditions. The effects of precoating dishes with basement membrane components will be tested, and the relationship of cell division to extracellular matrix synthesis will be explored. The potential relative roles of synthesis and degradation of basement membrane during repair of the alveolar lining will be evaluated. The total study will provide insight into the biosynthetic relationships between alveolar cells and the basement membranes which they make and adhere to.