TRACHEOBRONCHIAL GLYCOPROTEIN STRUCTURE

我们的目标是，在这项建议中，继续我们的研究工作， 化学和结构表征纯化的人 气管支气管糖蛋白 主要重点放在 分析主要的硫酸化低聚糖和主要的 分离的寡糖-寡肽亚基， 气管支气管糖蛋白 本提案的重点 强调硫酸化低聚糖的表征 因为主要是这个分数随着严重程度的增加而增加， 肺病。 气管支气管糖蛋白和分离的 来自患有各种形式的慢性炎症的患者的组分 阻塞性肺病（例如，慢性支气管炎，哮喘， 支气管扩张、肺泡蛋白沉积症和囊性纤维化（CF））将 研究，阐明和比较它们的结构，以获得 从不同的病理载体中了解到 气管支气管粘膜下腺改变其分泌 糖蛋白结构对慢性疾病的反应。 以来 从最初的提案开始，我们已经提出了实质性的， 在阐明许多硫酸化的结构方面取得了重大进展 低聚糖。 我们建议继续这项令人兴奋的工作， 除了加强我们的研究工作， 分离的不同寡糖-寡肽级分 气管支气管糖蛋白 气液色谱法和 质谱法将用于所有化学研究 其中还将包括几种新的分析技术 在初步提案期内制定的， 氨基和中性糖、氨基酸和羧酸盐。 我们对结构和相关功能的了解 气管支气管糖蛋白仍然很差。 过去 两年来，我们取得了重大发现;发现了几个 新的硫酸化结构。 我们逐渐开始瓦解 这些复杂的基本结构，大分子量 糖蛋白 继续这项研究是重要的， 我们可以了解这些细胞的结构和功能， 健康和疾病中非常重要的分子。

Our aim, in this proposal, is to continue our research efforts to chemically and structurally characterize purified human tracheobronchial glycoproteins. Primary emphasis is placed on analyzing the major sulfated oligosaccharides and the major oligosaccharide-oligopeptide subunits isolated from tracheobronchial glycoproteins. The focus of this proposal stresses the characterization of the sulfated oligosaccharides since it is primarily this fraction that increases with severity of a lung disease. Tracheobronchial glycoproteins and isolated fractions from patients suffering from various forms of chronic obstructive pulmonary disease (e.g., chronic bronchitis, asthma, bronchiectasis, alveolar proteinosis and cystic fibrosis (CF)) will be studied, their structures elucidated and compared so as to gain insight from different pathological vectors on how the tracheobronchial submusocal glands alter their secretory glycoprotein structure in response to chronic disease. Since the start of the initial proposal, we have made substantial and significant progress in elucidating the structures of many sulfated oligosaccharides. We propose to continue this exciting work, in addition to enhancing our research efforts in analyzing the different oligosaccharide-oligopeptide fractions isolated from tracheobronchial glycoproteins. Gas-liquid chromatography and mass spectrometry will be employed in all chemical investigations which will also include several new analytical techniques developed during the initial proposal period for the analysis of amino and neutral sugars, amino acids and carboxylates. Our knowledge of the structure and related functions of tracheobronchial glycoproteins is still very poor. Over the past two years we have made significant findings; discovering several new sulfated structures. We are gradually beginning to unravel the basic structure of these complex, large molecular weight glycoproteins. Continuation of this research is important so that we can understand the structure/function biochemistry of these very important molecules in health and disease.