MECHANISM OF POLYMERIZATION OF HB S

这项工作的目的是为了更好地了解这一机制 镰刀状HB的聚合和解聚。动力学和 将研究各种HBS聚合的热力学。至 阐明HB-S的核性质和分子性质的变化 和在预凝胶期的镰状和非镰状HbS的混合物 将用激光小角散射仪测量下 各种实验条件。申请者还将准备 Hb S的低聚物在成核过程中是交联的，以及 研究这些交联型低聚物是否可以作为原子核。 由镰状和非镰状HBS形成的混合HBS的作用将是 学习。申请者和同事最近在化学方面取得了成功。 用交联剂在分子内连接两条不同的链， 其中一种是富马酸二(3，5-二溴水杨基)酯。我会把自然的 Hb S、Hb X在聚合过程中的相互作用。我们对生物多样性的研究 镰状和非镰状HBs的聚合表明有许多 非镰刀形单体Hb与形成的核的相互作用类型 从HBS开始，我们将研究这些相互作用的性质。

The aim of this work is to provide a better understanding of the mechanism of the polymerization and depolymerization of sickle Hb. The kinetics and thermodynamcis of the polymerization of various Hbs will be studied. To elucidate the nature of nuceli, changes in molecular propperties of Hb S and mixtures of sickle and non-sickle Hbs during the pregelation period will be measured by a low angle laser light scattering photometer under various experimental conditions. The applicants will also prepare oligomers of Hb S which are cross-linked during the nucleation step and investigatae whether these cross-linked oligomers can act as nuclei. The role of hybrid Hbs formed from sickle and non-sickle Hbs will be studied. The applicant and co-workers recently succeeded in chemically connecting two unlike chains intramolecularly with corss-linking agents, one of which is bis (3,5 dibromosalicyl) fumarate. I will the nature of the interaction of Hb S, Hb X during polymerization. Our studies on the polymerization of sickle and non-sickle Hbs suggested that there are many types of interactions between non-sickle nonomeric Hb and nuclei formed from Hb S. The nature of these interactions will be studied.