DEVELOPMENT AND DIFFERENTIATION OF AIRWAY EPITHELIUM

识别气道细胞的特异性标志物是重要的 肺腺癌发生、分化研究的前提 气道细胞 我们计划在肺灌洗液中鉴定非血清蛋白， 产生针对这些蛋白质的单克隆抗体。 抗体将被检测 对于气道细胞的反应性和特异性， 免疫细胞化学 不同气道特异性标志物的可用性 细胞将促进细胞发育的研究。 细胞特异性 将使用标记物来研究许多因素对 气道细胞的发育，使用形态学，免疫细胞化学， 蛋白质的形态测定和定量作为方法学途径。 研究气道上皮细胞发育的调控可能是 在支气管肺发育不良患者的治疗中具有重要意义。 细胞特异性蛋白的鉴定和结构分析是 还可能提供对不同气道功能的了解， 细胞 了解气道细胞特异性蛋白质的功能， 为慢性阻塞性肺疾病的发病机制提供线索 和肺气肿 我们将分离细胞特异性蛋白质，并计划检查 它们的氨基酸序列，从长远来看， proteins. 这些基因的cDNA探针的可用性将是有价值的， 气道细胞特异性蛋白的基因调控研究。

Identification of specific markers for airway cells is an important prerequisite in studies of the development and differentiation of pulmonary airway cells. We plan to identify non-serum proteins in lung lavage and raise monoclonal antibodies to the proteins. The antibodies will be tested for their reactivity to and specificity for airway cells by immunocytochemistry. Availability of markers specific of different airway cells will facilitate the study of development of the cells. Cell specific markers will be used to study the effect of a number of factors on the development of airway cells, using morphology, immunocytochemistry, morphometry and quantitation of proteins as the methologic approaches. Studies of the regulation of development of airway epithelium may be important in the treatment of patients with bronchopulmonary dysplasia. Identification and structural analyses of the cell specific proteins is also likely to provide insight into the function of different airway cells. Knowledge of the function of specific proteins of airway cells may provide clues to the pathogenesis of chronic obstructive pulmonary disease and emphysema. We will isolate cell specific proteins, and plan to examine their amino acid sequence and, in the long run, isolate the genes for the proteins. Availability of cDNA probes for the genes will be valuable in studies of gene regulation of the airway cell specific proteins.